棉酚在肝癌细胞中作用机制的研究  

Study on the Mechanism of Activity on Hepatocarcinoma by Gossypol

作　　者：黄青红[1] 姚超[1] 胡徐庞[1] 王刚[1] 杨耿兵[1] 刘立[1] 钱程[1] 魏旭斌[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2012年第1期103-106,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(30872984)

摘　　要：棉酚是棉花中倍半萜(十五碳)的法尼基焦磷酸环化后合成的次生代谢产物。近来有研究显示棉酚具有抗肿瘤效果。本研究通过MTT、Hoechst染色、Western blot等生化技术开展棉酚对肝癌细胞系Bel-7404的增殖抑制及促凋亡作用研究。MTT实验结果表明5mmol棉酚即对肝癌细胞增殖具有强烈抑制作用,Hoechst染色表明5mmol/L棉酚即可对肝癌细胞具有促凋亡作用,Western blot结果表明棉酚能下调Bcl-2基因的表达。棉酚对肝癌细胞系Bel-7404具有很好的抑制增殖和促凋亡的效果。Gossypol is generated from sesquiterpene in cottons after thefarnesyl-pyrophosphoric is cyclized. For the past years there were evidences showing that the gossypol has the capacity of anti-tumor. The experiment studies the capacity of suppressing proliferation and promoting apoptosis in of gossypol in hepatoma cell line Bel-7404 via MTT Hoechst and West-blotting. The MTT shows that the gossypol has the activity of suppressing proliferation, the Hoechst shows that it promotes apoptosis in Bel-7404 and the Western-blot shows that it can reduce the expression of Bcl-2. The experiment offer the reference for the forward research and clinical application.

关 键 词：棉酚 肝癌 凋亡 Bd-2 

分 类 号：R73-362[医药卫生—肿瘤]