TGF-β1对肝癌细胞系Hep3B中干性相关基因表达的影响  

The Effect of TGF-β1 on Gene Expression Related to Stemness on Liver Cell Line Hep3B

作　　者：苏虹[1] 胡贲[1] 徐科[1] 何倩[1] 姚超[1] 钱程[1] 刘立[1] 

机构地区：[1]浙江理工大学生命科学学院新元医药研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2012年第1期125-128,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家973基金资助项目(2010CB529406)

摘　　要：有研究表明TGF-β1可以诱导诸多上皮来源的癌细胞和正常细胞发生EMT并使其功能发生改变。实验就TGF-β1对肝癌细胞系Hep3B的作用展开研究:通过CCK8检测TGF-β1对Hep3B细胞增殖的影响,RT-PCR实验检测TGF-β1处理后细胞中EMT及干性相关基因的表达变化。结果表明:TGF-β1对Hep3B细胞的增殖无抑制作用;TGF-β1处理Hep3B细胞6d后EMT相关基因的mRNA表达水平并无显著改变,但TGF-β1可上调Hep3B细胞干性基因Oct-4,Klf-4,Nanog,C-myc的表达,并下调分化基因albumin的表达。结果提示TGF-β1一定程度上影响肝癌细胞系的干性基因表达,但并不一定是以发生EMT为前提的。Studies have shown that TGF-β1 can induce the source of many cancer cells and normal epithelial cells place EMT, and its function changes. This study is TGF-β1 on the role of hepatocellular carcinoma cell line Hep3B. The authors detect the TGF-β influence on Hep3B cell proliferation by CCKS, as Hep3B with TGF-β1 treatment for six days, and detect the gene experssion proflie that related to EMT and stemness by qRT-PCR. The results show that. TGF-β1 has no inhibitory effect on the proliferation of Hep3B cells; Hep3B cells experience TGF-β1 treat after having no significant change in their EMT gene experssion, but TGF-β1 up-regulates Hep3B cells stem-related gene expression, and down their differentiation genes expression. This suggests that TGF-β1 to a certain extent affects the liver stem cell line gene expression, but not necessarily the prerequisite for EMT to occur.

关 键 词：TGF-Β1 HEP3B EMT 干性基因 

分 类 号：R73-362[医药卫生—肿瘤]