浙贝母精油化学成分GC/MS分析和抑菌活性检测  

Analysis of Chemical Composition by GC/MS and Antimicrobial Activities of Essential Oil from Fritillaria thunbergii Miq.

作　　者：曹跃芬[1] 竺锡武[1] 谭琳[1,2] 

机构地区：[1]浙江理工大学生物工程研究所,杭州310018 [2]湖南农业大学,长沙410128

出　　处：《浙江理工大学学报（自然科学版）》2012年第1期129-132,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省重中之重学科开放基金项目(SWYX0911);国家自然科学基金项目(31071729)

摘　　要：采用水蒸汽蒸馏法提取浙贝母精油,GC/MS分析法结合保留指数分析法鉴定精油化学成分,琼脂扩散法检测精油的抑菌活性。首次鉴定出浙贝母精油的15种化学成分,占精油总离子流色谱峰面积的97.48%,主要成分为正十六酸(53.46%),(E,E)-9,12-十八烷二烯酸甲酯(26.96%)和油酸(9.34%)。浙贝母精油对金黄色葡萄球菌和大肠杆菌均无抑制效果,对白色念珠菌有中等抑制作用。The essential oil from dried bulbs of Fritillaria thunbergii Miq. obtained by hydrodistil lation is analyzed by GC-MS together with Kovats retention index. The results show that major constituents of this oil are n-Hexadecanoic acid （53. 46%）, （E, E）-9, 12-Octadecadienoic acid （26.96%） and Oleic Acid （9.34%） and 15 chemical components are identified which represents 97. 5% of the total peak. The result tested by agar diffusion method shows that the oil has a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus.

关 键 词：浙贝母 精油 GC/MS Kovats保留指数 抑菌活性 

分 类 号：Q946.8[生物学—植物学]