水稻穗形态建成基因PMM1的遗传分析与初步定位  

Genetic analyses and preliminary mapping of a panicle morphogenesis mutant(PMM1) in rice

作　　者：李雪梅[1] 何宗顺[1] 余四斌[1] 陈国兴[1] 吴昌银[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第1期10-15,共6页Journal of Huazhong Agricultural University

基　　金：国家转基因专项(2008ZX08010-002);华中农业大学自主创新课题

摘　　要：在水稻粳稻品种中花11T-DNA插入突变体库中鉴定了3个穗形态突变体,它们均表现为植株半矮、叶夹角变小、一次枝梗轮生、复粒、粒长变短、粒宽变宽等突变表型。基因双突变杂交F1表型考查证明这3个突变体为等位突变体,T-DNA标签共分离检测表明这3个突变体的表型与T-DNA插入无关。通过与籼稻品种珍汕97配置3个杂交组合,由经典的孟德尔遗传分离比显示,突变性状受1对隐性基因(panicle morphological mutant 1,PMM1)控制。采用基因图位克隆的方法,已将基因PMM1定位在第4染色体长臂上的RM3866-1和X4(InDel)标记之间,其两侧物理图距为147kb左右。Three panicle morphogenesis mutants were identified from a T-DNA insertional mutant library of the japonica rice variety Zhonghua 11. All these mutants exhibited as semi-dwarf,reduced leaf angle,whorled primary branches, shortened and widened grains. The same mutant phenotype of F1 gen- erated by parallel crossing between three mutants verified that they were allelic. Cosegregation analysis of T-DNA tag confirmed that the mutant phenotypes were not caused by T-DNA insertion in the ge- nome. Three Fz mapping populations were generated by individually crossing mutants with Zhenshan 97. The segregation ratio of mutants and wild-types indicated that the mutant traits were controlled by a sin- gle recessive gene （panicle morphological mutant 1 ,PMM1）. Through map-based cloning, PMM1 was located between molecular markers RM3866-1 and X4 （InDel） on the long arm of chromosome 4,with physical region about 147 kb. This research will provide a foundation for further mapping and character- ization of the PMM1 gene in rice.

关 键 词：水稻 穗形态突变体 T-DNA标签 遗传分析 基因定位 图位克隆 

分 类 号：S511.503[农业科学—作物学]