大豆苗期氮高效和氮敏感资源的筛选研究  

Screening of Soybean Varieties with Different Nitrogen Efficiency at Seedling Stage

作　　者：郝青南[1,2] 王程[1] 陈水莲[1] 沙爱华[1] 单志慧[1] 陈海峰[1] 周蓉[1] 周新安[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,湖北武汉430062 [2]中国农业科学院研究生院,北京100081

出　　处：《大豆科学》2011年第6期910-915,920,共7页Soybean Science

基　　金：国家转基因重大专项重大课题(2008ZX08004-005)

摘　　要：以147个大豆品种为材料,采用温室苗期水培方法,观察各品种在低氮和正常供氮环境下叶片黄化程度,初步评价大豆品种苗期对低氮的反应。根据叶片黄化程度差异初步筛选出3个耐低氮品种(坡黄、铜山青大豆和徐豆8号),3个氮敏感品种(84-70、中品03-5358和龙豆7号)。通过对这6个品种氮积累相关指标的变异系数进行比较并结合各指标相关性分析结果,将干重、地上部分氮积累量、生物量增加值和氮吸收量作为判断耐低氮的指标,最终筛选出耐低氮型品种坡黄和氮敏感型品种84-70。Soybean requires more nitrogen than other major crops for sustaining seed growth.The nitrogen use efficiency（NUE）of soybean is different among varieties.In order to obtain the different NUE soybean varieties,a total of 147 varieties were screened in this study.Soybean seeds were germinated and grown in 1/2 times of Hoagland solution.Two N levels including low and normal levels were set in this experiment,and each level had three repetitions.The dry plant weight,stem length,root length biomass,nitrogen content and the amount of nitrogen absorption were investigated for choosing the indexes associated with NUE,and analyzed the relationship among these indexes.Based on the results of the primary screening,three low-N-tolerant varieties（Pohuang,Tongshanqing and Xudou 8）and three low-N-sensitive varieties（84-70,zhongpin 03-5358 and longdou 7）were selected and grown in nutrition solution at two N levels.The coefficient of variation could reflect the tolerant ability to low N-stress.After analyzed the coefficient of variation and correlation among the indexes,shoot nitrogen accumulation,increase in biomass,dry weight and the amount of nitrogen absorption were chosen as the indexes for evaluating the NUE of soybean varieties.The results indicated that ＂Pohuang＂ and ＂84-70＂ had the highest and lowest relative value,respectively.So ＂Pohuang＂ was tolerant and ＂84-70＂ was sensitive to low N-stress,respectively.

关 键 词：大豆 苗期 氮营养 筛选 

分 类 号：S565.1[农业科学—作物学]