食管鳞癌组织中VEGF-D表达及其与淋巴管密度和淋巴转移的关系  

Expression of VEGF-D in esophageal squamous cell carcinoma and the correlation with lymphatic vessel density and lymphatic metastasis

作　　者：叶武[1] 陈洪[2] 刘鹏飞[1] 刘兵团[1] 

机构地区：[1]东南大学附属江阴医院消化科,214400 [2]东南大学附属中大医院消化科,210009

出　　处：《国际消化病杂志》2011年第6期361-364,共4页International Journal of Digestive Diseases

摘　　要：目的研究食管鳞状细胞癌(ESCC)组织中血管内皮细胞生长因子D(VEGF-D)的表达情况及其与淋巴管密度(LVD)的关系,并探讨其与食管癌淋巴结转移的关系。方法免疫组化SABC法检测55例手术切除的食管鳞状细胞癌组织、癌旁组织、正常组织中淋巴管密度和VEGF-D的表达水平,对结果进行统计分析。结果 (1)食管癌组织内LVD为6.6582±2.0489个/HPF,显著高于癌旁组织(5.7527±1.4631个/HPF)及正常组织(5.5782±1.4553个/HPF)(P均<0.05);(2)食管癌组织中VEGF-D阳性表达率为43.6%,显著高于癌旁组织(16.4%)及正常组织(12.7%)(P均<0.05);(3)食管癌组织中VEGF-D表达强度与LVD之间呈正相关(r=0.9985,P<0.05);(4)食管癌患者中,淋巴结阳性组中VEGF-D阳性率(69.2%)及LVD(7.9308±2.0055个/HPF)均显著高于淋巴结阴性组(20.7%,5.5172±1.2857个/HPF)(P均<0.05)。结论 (1)食管癌组织中LVD增高VEGF-D呈高表达,且两者呈正相关,推测VEGF-D能诱导食管癌组织中的淋巴管生成;(2)LVD和VEGF-D表达强度检测与食管癌淋巴结转移相关。Objective To study the expression of vascular endothelial growth factor-D （VEGF-D） in esophageal squamous cell carcinoma （ESCC） and it＇s correlation with lymphatic vessel density（LVD）, and investigate the correlation with lymphatic metastasis of ESCC. Methods Fifty-five cases of esophageal squamous cell carcinoma with complete clinical data were included in this study. Immunohistochemical analysis was used to detect the expression of VEGF-D and LVD in carcinoma tissues, adjacent noncancerous tissues and corresponding normal tissues. Then the correlation of the expression of VEGF-D and LVD and lymphatic metastasis was analyzed by statistic methods. Results （1） LVD in carcinoma tissues （6. 6582± 2. 0489/HPF） was significantly higher than that in adjacent noncancerous tissues（5. 7527± 1. 4631/HPF） and corresponding normal tissues （5. 5782 ±1. 4553/HPF） （P〈0.05）. （2）The positive rate of VEGF-D in carcinoma tissues （43.6%） was significantly higher than that in adjacent noncancerous tissues （16.4%） and corresponding normal tissues （12.7%） （P〈0.05）. （3） There was positive correlation between the expression strength of VEGF-D and LVD（r = 0. 9985, P〈0. 05）. （4） The positive rate of VEGF-D and LVD in the positive lymph node group were significantly higher than those in the negative lymph node group （69.2% vs 20.7%, 7. 9308±2. 0055/HPF vs 5. 5172±1. 2857/HPF, P〈0. 05）. Conclusions （1） The Expression of VEGF-D and LVD are high in ESCC, and LVD overpress is associated with elevated VEGF-D, it is probably that VEGF-D can lead to lymphangiogenesis of ESCC. （2） LVD and the expression strength of VEGF-D in ESCC could be the important index for lymph node metastasis of ESCC.

关 键 词：食管鳞状细胞癌 血管内皮生长因子D 淋巴管密度 淋巴转移 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤]