人前列腺癌细胞特异性结合短肽在人体组织的分布  

The distribution of Human prostate cancer cell specific binding peptides in human tissues

作　　者：胡金秋[1] 李伟[2] 张丽红[2] 张国荣[1] 刘志晶[2] 董欣洁[2] 张玉辉[2] 李一雷[2] 

机构地区：[1]长春医学高等专科学校病理教研室,吉林长春130031 [2]吉林大学病理生物学教育部重点实验室,吉林长春130021

出　　处：《中国实验诊断学》2011年第12期2070-2073,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅研究项目资助课题(200905179);吉林省教育厅"十一五"科学技术研究项目资助课题(吉教科合字[2010]第460号)

摘　　要：目的观察人前列腺癌转移相关蛋白结合短肽在人体各组织的分布,为进一步探讨前列腺癌的可能转移机制提供实验证据,为该短肽在前列腺癌抗肿瘤导向治疗的应用提供形态学依据,探讨该短肽在未来临床应用的潜能。方法收集尸检标本12例,含正常人体组织16种,前列腺活检标本11例。通过免疫组织化学方法系统地鉴定此特异性短肽在人体各种组织、器官的分布以及在前列腺增生时前列腺组织中的表达情况。结果人前列腺癌细胞转移相关蛋白的特异性结合短肽与被覆上皮、肌组织、神经组织、结缔组织无结合;与大多数腺上皮无结合,但与胰岛细胞亲和阳性率为50%、胃底腺壁细胞亲和阳性率为60%;在良性前列腺增生时无表达,但在PC-3M细胞膜上有阳性表达。结论该短肽可与前列腺癌细胞表面特异性蛋白结合,而与大部分人体正常组织无结合,具有良好的特异性,证明该短肽具有临床应用的潜能。Objective To observe the distribution of the sequence CGWTPVI of human prostate cancer metastasis binding peptide in human tissues,in order to provide experimental evidence for transfer mechanism of prostate cancer and morphological evidence for the application of directed therapy of prostate cancer.Accordingly,investigate the potential of this peptide in clinical application.Methods autopsy specimens of 12 patients,including 16 kinds of normal human tissues and 11 cases of prostate biopsy specimens.Systematically identify the expression of the specific peptides in human normal tissues,organs and prostatic hyperplasia by immunohistochemistry.Results the peptides can not bind to lining epithelium,muscle tissue,nervous tissue,connective tissue and most glandular epithelium.But the affinity with the islet cells was 50%,and the affinity with parietal cells in fundic gland was 60%.The peptides have no expression in benign prostatic hyperplasia but the expression in PC-3M membrane.Conclusion the peptides can bind to prostate cancer cell surface specific protein,but have no combination with most human normal tissues.Therefore,these peptides have good specificity and potential clinical application.

关 键 词：PC-3M 特异性结合短肽 导向治疗 

分 类 号：R737.25[医药卫生—肿瘤]