5-烯丙基-7-二氟亚甲氧白杨素抑制肝癌细胞NF-κB表达的研究  

Antihepatoma Effect of 5-ally-7-gen-difluormethylenechrysin Through down Regulation Expression of NF-κB Protein,a Novel Chrysin Derivatives

作　　者：王宇[1] 周建国[2] 周秀田[2] 

机构地区：[1]长治医学院病理学教研室,046000 [2]南华大学肿瘤研究所

出　　处：《长治医学院学报》2011年第6期401-404,共4页Journal of Changzhi Medical College

基　　金：湖南省医药卫生科研基金(B2006-102)

摘　　要：目的:研究5-烯丙基-7-二氟亚甲氧白杨素(ADFMChR)抑制体外培养人肝癌SMMC-7721细胞增殖和诱导凋亡作用及机制。方法:体外培养SMMC-7721细胞,MTT比色法测定ADFMChR对SMMC-7721细胞增殖活性的影响;Western Blotting法分析ADFMChR对SMMC-7721细胞NF-kB蛋白表达活性的影响。结果:MTT比色法结果显示ADFMChR有效抑制SMMC-7721细胞增殖活性,呈剂量依赖性,其IC50值为3.56μM;Western Blot分析证实ADFMChR下调NF-κB蛋白表达,呈浓度和时间依赖性。结论:ADFMChR具有抑制人肝癌SMMC-7721细胞增殖和诱导细胞凋亡作用,其机制与NF-κB蛋白表达有关。Objective：To investigate the effect and mechanism of 5-ally-7-gen-difluormethylene chrysin（ADFMChR）,a novel chrysin derivatives,on the proliferation and apoptosis of human liver cancer（SMMC-7721）cell line in vitro.Methods： SMMC-7721cells was cultured in vitro.MTT assay was used to determine the effect of ADFMChR on the proliferation of SMMC-7721cells.Western blot was used to analyze expression of NF-κB protein in SMMC-7721cells.Results：ADFMChR significantly suppressed proliferation of SMMC-7721cell line in a dose-dependant manner.Its IC50was 3.56μM.Western Blotting indicated that expression of NF-κB protein decreased in a dose-and time-dependent manner.Conclusion：ADFMChR could inhibit proliferation and induce apotosis of human liver cancer SMMC-7721cell line.Its mechanism might be associated with down regulation expression of NF-κB protein.

关 键 词：肝癌 白杨素 5-烯丙基-7-二氟亚甲基白杨素 细胞凋亡 

分 类 号：R361[医药卫生—病理学]