4周龄Fmr1基因敲除小鼠的高架十字迷宫行为观察  

BEHAVIOURAL COMPARISION ON FMR1 KNOCKOUT MICE AT 4 WEEKS AGE IN ELEVATED PLUS-MAZE TEST

作　　者：刘国斌[1] 孙卫文[1] 黄月玲[2] 沈岩松[1] 戴丽军[2] 陈盛强[1] 李敏雄[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260 [2]广州医学院动物实验中心,广东广州510182

出　　处：《现代医院》2011年第12期12-14,共3页Modern Hospitals

基　　金：广东省自然科学基金项目(编号:815101700100005);广东省科技计划项目(编号:2008B030301371;2009B030801368;2009B060300018);广东省中医药管理局建设中医药强省科研基金项目(编号:2010206)

摘　　要：目的对4周龄的Fmr1基因敲除小鼠的高架十字迷宫行为进行观察。方法采用SMART软件对4周龄的Fmr1基因敲除小鼠(KO)和野生型小鼠(WT)进行高架十字迷宫行为观察,数据采用多因素方差分析处理。结果采用SMART软件分析发现:在高架十字迷宫实验中,与WT组小鼠比较,KO组小鼠的运动性、兴奋性、探索性明显增强,且KO组小鼠在开放区域活动时间,次数以及路程均明显高于WT组。结论 4周龄Fmr1基因敲除小鼠的行为异常,运动性和兴奋性较野生型小鼠增高。Objective This study was designed to identify Fmr1 knockout mice and compare the behavior difference at 4 weeks Age in Elevated Plus-maze test. Methods Fmr1 knockout mice were identified using the PCR and Elevated Plus-maze were used in the study.The data was analyzed with Multifactor Variance Analysis. Results Compared with non-treated WT mice,non-treated KO mice had more open arm entries,time spent and tracking in open arms in Elevated Plus-maze tests,it suggested that KO mice had Elevated Plus-maze behavior impairments. Conclusion Fmr1 knockout animals exhibited higher locomotor activity than WT in the Elevated Plus-maze task at 4 weeks Age.

关 键 词：高架十字迷宫 FMR1 基因敲除 小鼠 行为 

分 类 号：R363.1[医药卫生—病理学]