机构地区:[1]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150086 [2]黑龙江大学黑龙江省普通高等学校分子生物学重点实验室,黑龙江哈尔滨150080 [3]农业微生物技术教育部工程研究中心,黑龙江哈尔滨150500 [4]东北林业大学生命科学学院,黑龙江哈尔滨150040
出 处:《Agricultural Science & Technology》2011年第12期1881-1884,共4页农业科学与技术(英文版)
基 金:Supported by National Natural Science Foundation of China(31170466);2010 Science and Technology (guidance) Project from Heilongjiang Provincial Department of Education (11553079;11553080);Heilongjiang Provincial Natural Science Foundation (C200918);Heilongjiang Provincial Post-doctoral Start-up Research Fund (LBH-Q09022);High-level Personnel Support Project from Heilongjiang University (Ecological restorationteam Hdtd 2010-12)~~
摘 要:[Objective] This study aimed to explore the effects of phosphorus on the contents of vitamin E in different cultivars of soybean grains and find the optimum application amount of phosphorus for different genotypes of soybean cultivars,in order to increase the contents of vitamin E in soybean grains and improve the qualities.[Method] Three soybean cultivars were selected as experimental materials,including Heinong 48(high-protein cultivar),Heinong 37(intermediate cultivar) and Heinong 44(high-oil cultivar).The soybeans were planted in pots,with 0.033 g/kg soil of N and K2O,four phosphorus treatments were set,respectively applied with 0(P1),0.033(P2),0.067(P3) and 0.100(P4) g/kg soil of P2O5,and the total contents of vitamin E in different cultivars of soybean grains were determined by high-performance liquid chromatography method.[Result] The total contents of vitamin E in the same cultivar of soybean grains in P3 treatment were significantly higher than that in the other three treatments,the total contents of vitamin E in Heinong 37,Heinong 44 and Heinong 48 in P3 treatment had increased by 11.96%,16.55% and 14.02%,compared with the control;among the three soybean cultivars in P2 treatment,the content of vitamin E in Heinong 37 was the maximum;among the 12 treatment combinations,the total contents of vitamin E in Heinong 44 in P3 treatment was the maximum.The contents of vitamin E in three soybean cultivars significantly varied among the various cultivars and different phosphorus treatments.[Conclusion] Application of phosphorus could affect the contents of vitamin E in three soybean cultivars,appropriate application amount of phosphorus is advantageous to improve the contents of vitamin E in soybean grains.[目的]探索磷素对不同大豆品种籽粒维生素E含量的影响,寻找不同基因型大豆品种的最佳施磷水平,以期提高大豆籽粒维生素E的含量,改善其品质。[方法]选用黑农48(高蛋白品种)、黑农37(中间型品种)、黑农44(高油品种)3个大豆品种作为试验材料,采用盆栽,在每千克土壤施N和K2O各为0.033g基础上,设4个施磷处理,即每千克土壤分别施0(P1)、0.033(P2)、0.067(P3)、0.100(P4)gP2O5,最后采用高效液相色谱法测定不同大豆品种籽粒总维生素E的含量。[结果]同一品种大豆P3处理下其籽粒总维生素E含量显著高于P1、P2、P4处理,黑农37、黑农44、黑农483个品种大豆P3处理籽粒总维生素E含量分别比对照组增加11.96%、16.55%、14.02%;在P2处理下,3个品种中的黑农37籽粒维生素E含量最高;在12个处理组合中黑农44的P3处理总维生素E含量最高,3个大豆品种维生素E含量在品种间和施磷处理间差异显著。[结论]试验中施磷对3个大豆品种的维生素E含量有影响,适宜的施磷量有利于提高大豆籽粒的维生素E含量。
关 键 词:SOYBEAN Phosphorous level Vitamin E
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