COX-2抑制剂对PC-3细胞的放射增敏作用研究  

Study of enhancement of radiosensitivity in pancreatic carcinoma cell line PC-3 by cyclooxygenase-2 inhibitor

作　　者：宋轶鹏[1] 臧茹琨[1] 马金波[1] 

机构地区：[1]青岛大学附属烟台毓璜顶医院放疗科,264000

出　　处：《中国老年保健医学》2011年第6期18-21,共4页Chinese Journal of Geriatric Care

基　　金：山东省科技发展项目编号2004GG3202019

摘　　要：目的应用选择性环氧合酶-2(COX-2)抑制剂NS-398作用于胰腺癌细胞株PC-3,观察其放射增敏作用,并对其可能增敏机制进行初步探讨。方法体外培养胰腺癌细胞株PC-3;以流式细胞术(FCM)单抗标记技术检测PC-3细胞的COX-2表达水平;以MTT实验检测细胞增殖抑制情况及NS-398对细胞放射敏感性的影响;以FCM分析细胞周期变化,以RT-PCR分析细胞周期相关蛋白P21表达变化。结果 FCM显示PC-3细胞存在COX-2的异常高表达;MTT实验显示NS-398可显著增强PC-3细胞的放射敏感性;FCM显示NS-398联合放射组G0-G1期比率上调;RT-PCR显示NS-398联合放射组细胞内P21mRNA表达上调。结论选择性COX-2抑制剂NS-398可增强胰腺癌细胞PC-3的放射敏感性,其机制可能与上调P21mRNA表达,进而诱导细胞周期阻滞于G0-G1期相关。Objective To study the radiosensitizing effect of NS -398, a selective cyclooxygenase -2 inhibitor, on pancreatic carcinoma cell line PC -3 in vitro and investigate possible mechanisms. Methods Pancreatic carcinoma cell line PC -3 were cultured in RPMI1640. flow cytometry analysis（FCM） was used to detect the express of COX -2. MTT assay was performed to evaluate the cytotoxicity of NS - 398 and its radiosensitizing effect on PC - 3 cell. FCM was applied to explore the change of cell cycle distribution. RT - PCR was applied to detect P21 mRNA expression change after the application of NS - 398 and radiation. Results FCM showed positive COX - 2 express into PC - 3 cell. MTT assay indicated that NS - 398 could inhibit the proliferation of PC - 3 cell and enhance its radiosensitivity significantly. Flow eytometry analysis showed that treatment with NS -398 and radiation can increase the ratio of G0 - G1. RT - PCR analysis indicated that expression of P21 mRNA increased after treated with NS - 398 and radiation. Conclusion Our results demonstrate that NS - 398 can enhance the radiosensitivity of pancreatic carcinoma PC - 3 cell. Its mechanism may be associated with up regulation the expression of P21 mRNA and induced arrest of G1.

关 键 词：环氧合酶-2抑制剂 辐射增敏 细胞周期P21 胰腺癌细胞 

分 类 号：R735.9[医药卫生—肿瘤]