斑鳢嗜水气单胞菌病病原的鉴定及其病理组织学的研究  

Histopathology and identification of pathogen Aeromonas hydrophila in diseased snakehead Channa maculata

作　　者：陈言峰[1] 马文山[1] 陈文慧[1] 梁日深[1] 廖富蘋[1] 邹记兴[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《大连海洋大学学报》2011年第6期514-520,共7页Journal of Dalian Ocean University

基　　金：国家星火计划项目(2008GA780002);广东省教育部产学研结合项目(2011B090400270);清远市科技计划项目(2011C011106009)

摘　　要：从患病斑鳢Channa maculata体内分离到一株细菌(FS20100810),经人工感染试验证实为该病的病原菌。该菌株兼性厌氧,为革兰氏阴性杆菌,能运动,无芽孢,无荚膜,能发酵葡萄糖、蔗糖、麦芽糖、甘露醇、七叶苷,氧化酶反应、接触酶反应、吲哚试验和M.R.试验均为阳性。该菌株对斑鳢的半致死浓度为6.83×105 cfu/mL。16S rRNA基因序列与嗜水气单胞菌Aeromonas hydrophila的同源性为99.6%。药敏试验结果显示,该菌株对氧氟沙星、菌必治、先锋噻肟等抗生素敏感,对复方新诺明等中度敏感,对苯唑青霉素、青霉素G、氨苄青霉素耐药。通过对人工感染的斑鳢进行组织切片观察,发现病鱼的鳃、心肌、肝脏、肾脏、脾脏、肠道等器官组织均有明显的病理变化。A pathogen（FS20100810） was isolated from ulcered snakehead, Channa maculata, which was proved to be the pathogenic bacterium of the disease by re-infection experiments. The strain was found to be gram negative, anaerobic, bacilliform in shape and motile. It had no capsule and gemma,and utilized glucoses, sucrose, maltose, mannitol, esculin as energy and its oxidase reaction, catalase reaction, indole and M.R. test were positive. The half lethal concentration value for the strain to the snakehead was 6.83 ×10^5 cfu/mL. Homology analysis showed that it had the high similarity to Aeromonas hydrophila with 99.6% similarity. The drug sensitivity showed the strain was sensitive to ofloxacin, ceftriaxone, and Cefotamine was intermediately sensitive to complex sinomin and was resistant to oxazocilline, penicillin G and ampicillin. There were obvious pathological changes in the tissues of the gills, cardiac muscles, livers, kidneys, spleens and intestines in the snakeheads challenged by strain FS20100810 by pathohistological observation.

关 键 词：斑鳢 嗜水气单胞菌 16S RRNA 毒力因子 组织病理 

分 类 号：S941.42[农业科学—水产养殖]