红外光谱法对贮藏白花丹参各部位的分析与评价  

Analysis and Evaluation of Storage Time on the Quality of Different Parts of Salvia miltiorrhiza f.alba by Tri-steps Infrared Macro-fingerprint Method

作　　者：康玉秋[1] 郭庆梅[1] 赵启韬[2] 周凤琴[1] 孙素琴[3] 

机构地区：[1]山东中医药大学药学院,济南市250355 [2]山东中医药大学基础医学院,济南市250355 [3]清华大学化学系,北京市100084

出　　处：《光散射学报》2011年第4期387-395,共9页The Journal of Light Scattering

基　　金：山东省优秀中青年科学家科研奖励基金项目(2006BS03047)

摘　　要：采用傅里叶变换红外光谱、二阶导数谱和二维相关红外光谱三级鉴别法对贮藏白花丹参的根、茎、叶、花等部位及各部位的水溶性提取物、脂溶性提取物进行分析与评价研究。结果表明,贮藏5年各部位与当年采收的同部位的一维光谱非常相似,但二阶导数谱中峰位和峰强的差异明显。1800～1350cm-1范围内的二维相关谱中,当年的根和花的自动峰数目多于贮藏5年的,而当年的茎和叶中自动峰数目少于贮藏5年的。不同部位水溶性提取物和脂溶性提取物的分析结果进一步明确了白花丹参经贮藏5年后其主体化学成分基本未变,但各成分的含量略有不同。可见红外光谱三级鉴别法为贮藏后药材的细微差异分析和评价提供了一种快速、全面和客观的方法和手段。The Tri-Step identification technique including Fourier Transform Infrared Spec- troscopy （FTIR）, Secondary Derivative IR Spectra and Two-dimensional Correlation Spec- troscopy （2D-IR） was applied to analyze and evaluate the effect of storage time on the dif- ferent parts of Salvia miltiorrhiza f. alba. The crude drugs of Salvia miltiorrhia f. alba and their different extracts of the root, stem, leaf and flower were all referred. Similar re- sults were obtained from single dimensional FTIR for the different parts of Salvia miltior rhiza f. alba stored different time, while the shape and intensity of the peaks in the secondary derivative IR spectra of the different Darts showed distinct differences. In the 2D-IR related spectrum range of 1800~1350 cm-1 , the number of autopeaks in root and flow- er stored 0 years were more than that stored 5 years, while number of autopeaks in stem and leaf stored 0 years were less than that stored 5 years. The spectra of water-soluble ex- tracts and ethanol-soluble extracts from different parts of Salvia miltiorrhiza f. alba stored different time was analyzed, and results showed that the main compounds remained invariant, while the percentages of constituents were different. Therefore, Tri-Step iden- tification technique was an express and comprehensive approach to analyze and evaluate the imperceptible differences among different parts of the medicinal herbs stored for years ob- jectively.

关 键 词：白花丹参 贮藏 水溶性提取物 脂溶性提取物 红外光谱三级鉴别 

分 类 号：O657.3[理学—分析化学]