18β-GA对人高转移卵巢癌HO-8910PM细胞增殖、黏附和侵袭能力的影响  

Effects of 18β-glycyrrhetinic acid on the proliferation,adhesion and invasion in HO-8910PM cells

作　　者：陈杰[1] 庞江琳[2] 覃燕梅[1] 梁念慈[1] 

机构地区：[1]广东医学院,广东湛江524023 [2]广东医学院附属医院

出　　处：《山东医药》2011年第47期12-14,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(39870900);广东省重点学科基金资助(Gx0301)

摘　　要：目的观察18β-甘草次酸(18β-GA)对人高转移卵巢癌HO-8910PM细胞增殖、黏附和侵袭能力及黏附斑激酶(FAK)、基质金属蛋白酶(MMP)-9表达的影响。方法将18β-GA作用于HO-8910PM细胞,采用MTT法检测HO-8910PM细胞增殖抑制率;细胞黏附试验检测细胞黏附能力;Transwell chamber法检测细胞侵袭能力;Westernblot法检测细胞FAK、MMP-9蛋白表达水平。结果 18β-GA明显抑制高转移卵巢癌细胞HO-8910PM的生长增殖、黏附和侵袭能力;Western blot法检测表明药物能下调FAK、MMP-9蛋白的表达。结论 18β-GA可抑制HO-8910PM细胞黏附、侵袭,其机制可能是通过下调FAK、MMP-9蛋白表达而进行的。Objective To investigate the effects of 18β-glycyrrhetinic acid（18β-GA）on the proliferation,adhesion,invasion and the expression of FAK,MMP-9 of human highly metastatic ovarian carcinoma HO-8910PM cells.Methods HO-8910PM cells were treated with 18β-GA,then MTT assay was used to determine the growth inhibition;Transwell chamber assay was performed to determine the effect on invasion of the cells by 18β-GA;cell adhesion assay was performed to detect the adhesion of HO-8910PM;Western blot analysis was performed to detect the protein expreesion of FAK and MMP-9.Results 18β-GA inhibited the growth,proliferation,adhesion,invasion in cells;18β-GA also down-regulated the expression of FAK,MMP-9 protein.Conclusion 18β-GA can inhibit the proliferation,adhesion and invasion of HO-8910PM cells,the mechanism may be involved in the reduction of the expression level of FAK,MMP-9.

关 键 词：18Β-甘草次酸 黏附斑激酶 基质金属蛋白酶-9 卵巢肿瘤 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]