Mina53、Ki-67在中国人大肠癌中的表达及其与临床病理学之间的关系  

Expression and Clinical Significance of Mina53 and Ki-67 in colorectal carcinoma

作　　者：李爽[1] 陈元[2] 

机构地区：[1]长江大学附属第一医院（荆州市第一人民医院）肿瘤诊疗中心,荆州434000 [2]华中科技大学附属同济医院肿瘤科

出　　处：《实用肿瘤学杂志》2011年第6期537-543,共7页Practical Oncology Journal

摘　　要：目的 检测Mina53、Ki-67在大肠癌中的表达水平,研究其与临床病理学之间的关系,判断其表达对预后的意义,为大肠癌的早期诊断预防和治疗提供分子生物学依据.方法 收集长江大学附属第一医院,荆州市第一人民医院2004年12月-2006年12月大肠癌手术治疗的标本82例,应用免疫组化SP法检测组织切片中Mina53、Ki-67的表达,并与临床特征、病理分级等因素进行相关性分析.结果 82例大肠癌组织标本中,Mina53以及Ki-67均为核染色,Mina53阳性表达率为78.05%（64/82）,Ki-67阳性表达率为80.49%（66/82）;Spearman相关性检验显示,Mina53表达与Ki-67表达呈正相关（P=0.000）,Mina53、Ki-67表达水平与分期相关,P值分别为0.029、0.037,Mina53、Ki-67表达水平越高,分期越晚.与年龄、肿块大小以及病理学分级无关;Cox回归分析显示,Mina53表达、术后分期与预后相关,P值分别为0.039、0.000,而Ki-67表达、病理分级、肿块大小与预后无关.结论 Mina53、Ki-67在大肠癌细胞中呈特异性高表达,且Mina53表达水平与Ki-67表达水平明显相关,两者的高表达提示肿瘤易出现淋巴结转移及远处转移,Mina53可能成为判断大肠癌预后的因素之一.Objective To investigate the expression of MinaS3 and Ki -67 in the human colorectal car- cinoma, and the correlation between the expression and the clinicopathology, study the clinical significance of Mi- naS3 and Ki -67 in colorectal carcinoma, and afford a new molecule biological evidence and method for earlier diagnosis, prevention and therapy of colorectal cancer. Method 82 specimens of different colorectal carcinoma tissue were collected by surgery in the first people＇s hospital in Jingzhou between Dec. 2004 and Dec. 2006. All of the specimens were detected with the immunhistochemical method of SP by the MinaS3 antibody and the Ki -67 antibody, respectively. The relationship between the expression of MinaS3, Ki - 67 and the clinicopathology were analyed by SPSS13.0. Results The positive rates of the expression of MinaS3 and Ki - 67 were 78.05% （64/ 82 ） and 80.49% （ 66/82 ） in human colorectal carcinoma, respectively, which were significant higher than those in adjacent nonneoplastic tissues. The expression of MinaS3 and Ki -67 was related to the Duke＇s stage, the P value is 0.029 and 0.037, respectively. There was no obvious correlation between the expression and the age, tumor size and the pathological grade;univariate analysis of Cox proportional hazards regression model revealed that the ex- pression of MinaS3 and the stage were relevant to prognosis, the P value was 0. 039 and 0. 000, respectively. Condusion Overexpressions of MinaS3 and Ki -67 were found in the eolorectal carcinoma compared with no expression in the adjacent nonneoplastic tissues, and this maybe illustrate the invasion and metastasis of the tumor. MinaS3 maybe serve as a prognostic factor of colorectal carcinoma.

关 键 词：大肠癌 免疫组化 MINA53 KI-67 

分 类 号：R735.34[医药卫生—肿瘤]