菊花中总黄酮的提取工艺比较  

The Comparation of the Extraction Methods for Total Flavonoids of the Chrysanthemum

作　　者：刘小锐[1] 申伟[1] 梅林[2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]中国人民解放军后勤工程学院油料应用于管理工程系,重庆401311

出　　处：《西南师范大学学报（自然科学版）》2011年第6期110-113,共4页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：为优化超声波法和酶解法提取菊花总黄酮的工艺,并比较其工艺条件,选择因素水平,采用L9(34)正交设计优化提取工艺,以芦丁为对照品,用紫外分光光度计分析其提取率.结果表明,酶解法最佳工艺条件为:25倍70%的乙醇溶液,酶添加量0.5%,于45℃水浴下酶解150min,得率为5.60%;超声波法最佳工艺条件为:10倍70%的乙醇溶液超声40min,提取3次,得率为4.61%.酶解法的得率比超声波法的高21.5%.Objective To optimize the Ultrasonic Extraction and Enzymatic Hydrolysis Extraction, and to compare these two methods. Methods With the Rutin as the reference substance and ultraviolet spectro- photometer for analyzing the extraction rate, the extraction process is optimized after the level of factor is chosen and an orthogonal designing method L9 （3^4） is adopted. Results The superior process conditions for the Enzymatic Hydrolysis Extraction are. 25 Ethanol solution （70%）, with cellulose （0.5%）, constant temperature （45 ℃）, water bath for 150 minutes. Extraction rate is 5.60%. The superior process conditions for the Ultrasonic Extraction are： 10 Ethanol solutions （70%）, ultrasound for 40 minutes, extract for 3 times. Extraction rate is 4.61%. Conclusion The extraction rate by the Enzymatic Hydrolysis Extraction is 21.5% higher than the rate by the Ultrasonid Extraction.

关 键 词：菊花 总黄酮 酶解法 超声法 提取 正交设计 

分 类 号：TQ460.72[医药卫生—药物分析学]