烟草青枯病拮抗真菌烟曲霉发酵条件的优化  

Optimization of Fermentation Conditions of Fungus Inhibiting Ralstonia solanacearum by Orthogonal Test

作　　者：周晓见[1,2] 査鑫垚 董夏伟[1] 缪莉[1,2] 董昆明[1,2] 靳翠丽[1,2] 

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]扬州大学海洋科学与技术研究所,江苏扬州225127

出　　处：《河南农业科学》2011年第12期93-97,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(41076097;41006097);教育部科学技术研究重点项目(211065);江苏省自然科学基金项目(BK2010322);江苏省环境材料与环境工程重点实验室开放课题(K090027;K090025;K090026;K090028);扬州大学"新世纪"人才工程

摘　　要：烟曲霉(Aspergillus fumigatus)对烟草青枯病菌(Ralstonia solanacearum)有稳定的拮抗作用,在验证其发酵液对烟苗生长无害的前提下,利用四因素(发酵温度、发酵时间、接种量、装液量)三水平三重复正交试验,测定该真菌的最佳发酵条件。结果表明,烟曲霉的最佳发酵条件为:发酵温度28℃、发醇时间96h、接种量5%、装液量50mL(150mL容器),此时真菌粗提物产量达301.3μg,且抑菌活性最高,青枯病菌OD630平均值为0.370。试验还证明,装液量和发酵温度是影响烟曲霉发酵效果的主要因素。One strain of fungus inhibiting Ralstonia solanacearum was previously selected among 59 strains,which were isolated from healthy soil collected in Chongqing tobacco-growing region.The rDNA ITS sequence indicated that the active fungus was Aspergillus fumigatus.In this study,the fermentation liquid of A.fumigatus was firstly proved to be harmless to tobacco seedlings,and then its fermentation conditions were optimized according to orthogonal experimental design for enhanced antimicrobial effect.The results suggested that the optimal cultivation conditions were temperature of 28℃,cultivation time of 96h,inoculation volume of 5% and medium volume of 50mL in 150mL fermentation flask.Under those conditions,the quality of crude extract was 310.3μg,and the crude extract had the highest inhibition activity,when the average OD630 of Ralstonia solanacearum was 0.370.The medium volume and culture temperature were the major factors that affected the productivity of A.fumigatus product.

关 键 词：烟草青枯病菌 拮抗作用 真菌 烟曲霉 正交试验 发酵条件 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]