柳叶蜡梅化学成分及其抗氧化活性研究  

Constituents of Chimonanthus salicifolius and Their Antioxidant Activity

作　　者：潘心禾 史小娟[2] 张新凤[2] 斯金平[2] 

机构地区：[1]浙江省丽水林业科学研究院中药材研究所,浙江丽水323000 [2]浙江农林大学亚热带森林培育国家重点实验室培育基地天然药物研究开发中心,浙江临安311300

出　　处：《中国实验方剂学杂志》2012年第1期99-102,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省重大科技计划项目(2009C02005)

摘　　要：目的:研究柳叶蜡梅Chimonanthus salicifolius叶中的化学成分及其抗氧化活性。方法:采用各种柱色谱法进行分离纯化柳叶蜡梅叶中的化学成分,采用理化性质和NMR等波谱学方法鉴定化合物结构;采用ABTS法评价各化合物的抗氧化活性。结果:从柳叶蜡梅叶乙醇提取物中分离得到7个化合物,分别为7-羟基-6-甲氧基香豆素(1),莨菪亭-7-O-β-D-葡萄糖苷(2),山萘酚-3-O-β-D-葡萄糖苷(3)、山萘酚-3-O-芸香糖苷(4)、山萘酚(5)、槲皮素(6)和大黄素-8-O-β-D-葡萄糖苷(7)。抗氧化活性结果表明化合物1,5,6具有较好的清除抗氧化活性(ABTS)自由基的作用,其半数抑制率(IC50)分别为8.72,1.62,0.563 mmol.L-1。结论:化合物2,3,7均是首次从蜡梅属植物中分离得到,部分化合物具有较好的抗氧化活性。Objective：To study the constituents from Chimonanthus salicifolius and their antioxidant activities.Method：Constituent isolation and purification was carried by kinds of column chromatography,and their structures were elucidated on the basis of spectral data analysis.ABTS method was used to evaluate the free radical scavenging activity of the isolated compounds.Result：Seven compounds（1-7） were isolated and their structures were identified as follows：scopoletin（1）,scopoletin-7-O-β-D-glucoside（2）,kaempferol-3-O-β-D-glucoside（3）,kaempferol-3-O-β-D-rutinoside（4）,kaempferol（5）,quercetin（6）,emodin-8-O-β-D-glucopyranoside（7）.The compounds 1,5 and 6 showed higher antioxidant activity than positive control trolox with IC50 values 8.72,1.53,0.563 mmol·L-1 respectively.Conclusion：Compounds 2,3 and 7 were isolated from this genus for the first time.Some compounds had the significant antioxidant activity.

关 键 词：柳叶蜡梅 化学成分 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]