双黄连注射剂中黄芩苷致敏原性的研究  

Study of Allergenicity of Baicalin in Shuanghuanglian Injection

作　　者：曾姣丽[1] 贺福元[1,2] 唐昱[1] 段晓鹏[1] 王海琴[1] 谢相贵[1] 包小燕[1] 

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]湖南中医药大学药学院中药药性与药效国家中医药管理局重点实验室,长沙410208

出　　处：《中国实验方剂学杂志》2012年第1期102-106,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073142);湖南省教育厅重点项目(09A068);湖南省自然基金重点项目(11JJ2055)

摘　　要：目的:研究双黄连注射剂中黄芩苷的致敏原性。方法:通过运用酶联免疫双抗夹心和免疫指纹图谱2种方法结合对双黄连注射剂中黄芩苷的致敏原性进行研究。结果:运用酶联免疫双抗夹心法检测黄芩苷的致敏性,结果呈阳性反应;运用免疫指纹图谱法检测黄芩苷的致敏性,致敏率为86.28%。结论:通过运用以上方法结合确定了双黄连注射剂中黄芩苷为致敏原,建立了快速筛查中药注射剂中致敏原的分析方法。Objective：To study the allergenicity of baicalin in Shuanghuanglian injection.Method：The allergenicity of baicalin in Shuanghuanglian injection was studied by using combination of two methods of double-antibody sandwich ELISA and immune fingerprint.Result：The allergenicity of baicalin was detected by using double-antibody sandwich ELISA;the result is positive;the allergenicity of baicalin was detected by using immune fingerprint;its sensitization rate was 86.28%.Conclusion：Baicalin was determined as allergen in Shuanghuanglian injection by using the above method,which can establish a method of analysis to screen allergens rapidly in traditional Chinese medicine injection.

关 键 词：黄芩苷 致敏原 酶联免疫 指纹图谱 

分 类 号：R283.6[医药卫生—中药学]