E钙黏蛋白、c-Src蛋白在胃癌中的表达及其与侵袭转移的关系  

Expression of E-cadherin,c-Src in gastric carcinoma and relationship with invasion and metastasis

作　　者：刘江惠[1] 姜忠彩[1] 郭建文[1] 左连富[1] 

机构地区：[1]河北医科大学第四医院肿瘤研究所,河北石家庄050011

出　　处：《临床荟萃》2012年第1期27-30,F0002,共5页Clinical Focus

基　　金：河北省自然科学基金(303517)

摘　　要：目的通过检测E钙黏蛋白(E-cadherin,E-cad)和c-Src蛋白在胃癌中的表达,探讨其在肿瘤转移和侵袭过程中的作用。方法采取胃癌组织50例,正常胃黏膜10例;应用免疫组织化学链霉素抗生物素-过氧化酶连接(SP)法检测E-cad、c-Src蛋白的表达。结果①胃癌组织中E-cad的阳性表达率为46.0%(23/50),显著低于正常黏膜100%(10/10)(P<0.01)。转移组E-cad阳性表达率35.3%(12/34)明显低于非转移组68.8%(11/16)(P<0.05)。与临床分期、浸润分级的关系显示,Ⅲ～Ⅳ期和T3～T4级癌组织中E-cad表达分别明显低于Ⅰ～Ⅱ期和T1～T2级(均P<0.05)。E-cad表达还与组织学分级相关,胃癌分化程度低,其阳性表达率也低,分化程度高,阳性表达率也高(P<0.05)。②c-Src蛋白在Ⅲ～Ⅳ期的表达明显高于Ⅰ～Ⅱ期(P<0.05);T3～T4级c-Src蛋白表达高于T1～T2级(P<0.05)。结论 c-Src蛋白在胃癌中均呈高表达,而E-cad则反之。E-cad表达减弱与胃癌侵袭转移潜能相关,可作为胃癌侵袭转移的生物学标志之一。Objective To investigate the expression of E-cadherin（E-cad）,c-Src and their relationship with invasion and metastasis in gastric carcinoma.Methods 50 gastric carcinoma samples were analyzed and 11 normal gastric mucosa samples were studied as controls.SP method was used to detect the expression of E-cad,c-Src of all samples.Results ①The positive expression of E-cad in gastric carcinoma group（46.0%,23/50） was significantly lower than that in normal gastric mucosa group（100%,10/10）（P0.01）.The positive rate of E-cad in non-metastatic group（35.3%,12/34） was higher than that in metastatic group（68.8%,11/16）（P0.05）.The expressions of E-cad in stages Ⅰ-Ⅱ and T1-T2 were significantly higher than those in Ⅲ-Ⅳ and T3-T4（all P0.05）.The significant correlationship was observed between E-cad expression and histological grade（P0.05）.The positive rate of E-cadherin in poorly differentiated group was significantly lower than that in well differentiated group.②The expressions of c-Src in stages Ⅲ-Ⅳ and T3-T4 were significantly higher than those in Ⅰ-Ⅱ and T1-T2（all P0.05）.Significant correlationship was observed between c-Src expression and histological grade.Conclusion The over-expression of c-Src was examined in gastric carcinoma,E-cad on the opposite.The lower expression of E-cad was possibly related to metastasis and invasion of carcinoma,E-cad may also prove as a useful marker for determining the biological aggressiveness of gastric carcinoma.

关 键 词：胃肿瘤 肿瘤转移 癌基因蛋白质类 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]