异丙酚对抑郁大鼠电休克处理后海马CaMKⅡα活性的影响  被引量:1

Effects of propofol on CaMK Ⅱα activity in hippocampus in mentally depressed rats after electroconvulsive therapy

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作  者:李晓[1] 闵苏[1] 李炜[1] 罗洁[1] 魏珂[1] 黎平[1] 刘小滨[1] 綦欣竹[1] 

机构地区:[1]重庆医科大学附属第一医院麻醉科,400016

出  处:《中华麻醉学杂志》2011年第11期1302-1305,共4页Chinese Journal of Anesthesiology

基  金:国家自然科学基金(30972831);重庆市教育委员会科学技术研究项目(KJ090309)

摘  要:目的探讨异丙酚对抑郁大鼠电休克处理后海马钙离子/钙调蛋白依赖性蛋白激酶Ⅱα(CaMKⅡα)活性的影响。方法健康成年雄性SPF级SD大鼠,体重180~220g,2.3月龄,采用慢性轻度不可预见性应激法建立抑郁模型。取模型制备成功的大鼠40只,采用随机数字表法,将其随机分为4组(n=10):抑郁组(D组)腹腔注射生理盐水8ml/kg;单纯异丙酚组(P组)腹腔注射异丙酚80mg/kg;单纯电休克组(E组)腹腔注射生理盐水8ml/kg后实施电休克处理;异丙酚联合电休克(DPE)组腹腔注射异丙酚80mg/kg,待翻正反射消失后行电休克处理。以上处理每天1次,连续7d。于处理前1d和处理后1d采用糖水偏好实验评价抑郁状态。于处理前1d和处理后3d采用Morris水迷宫实验检测学习记忆能力。于Morris水迷宫实验完成后1d,采用免疫组织化学法检测大鼠海马磷酸化CaMKⅡα(pCaMKⅡα)及CaMKUa的表达,计算pCaMKⅡα/CaMKⅡα比。结果与D组比较,E组、DPE组糖水偏好比升高,E组逃避潜伏期延长,空间探索时间缩短,海马CaMKⅡα和pCaMKⅡα表达下调,pCaMKⅡα/CaMKⅡα比降低,DPE组逃避潜伏期缩短,空间探索时间延长,pCaMKⅡα表达上调(P〈0.05);与E组比较,DPE组逃避潜伏期缩短,空间探索时间延长,CaMKⅡα和pCaMKⅡα表达上调,pCaMKⅡα/CaMKⅡα比升高(P〈0.05)。结论异丙酚减轻抑郁大鼠电休克处理后认知功能损害的机制可能与增强海马CaMKⅡα的活性有关。Objective To investigate the effects of propofol on ealeium/calmodulin-dependeut protein ki- naseⅡα ( CaMK Ⅱα) activity in hippocampus in mentally depressed rats after eleetroconvulsive therapy (ECT). Methods Healthy adult male SD rats aged 2-3 months weighing 180-220 g were used in this study. Mentally depressed model was induced by chronic unpredictable mild stress. Forty mentally depressed rats were randomly divided into 4 groups (n = 10 each): mental depression group (group D), propofol group (group P), ECT group (group E) , propofol + ECT group (group DPE). Groups D and P received intraperitoneal normal saline 8 ml/kg or propofol 80 mg/kg once a day for 7 consecutive days respectively. Group E received ECT once a day for 7 consecutive days. Group DPE received propofol 80 mg/kg + ECT once a day for 7 consecutive days. Sucrose preference test was performed at 1 d before and 1 d after treatment, and Morris water maze test was performed at 1 d before and 3 d after treatment . The rats were sacrificed after Morris water maze test, and hippocampi were removed for determination of CaMK Ⅱαand phosphorylated CaMKⅡα( pCaMK Ⅱα )expression, and pCaMK Ⅱα/CaMK Ⅱαratio was caculated. Results Compared with group D, the sucrose preference percentage was significantly increased in groups E and DPE, the escape latency prolonged and space exploration time shortened, and the expression of CaMKⅡα and pCaMK Ⅱα down-regulated, pCaMK Ⅱα/CaMK Ⅱα ratio decreased in group E, the escape latency was significantly shortened and space exploration time prolonged, and the expression of pCaMK Ⅱα up-regulated in group DPE (P 〈 0.05). Compared with group E, the escape latency was significantly shortened, space exploration time prolonged, and the expression of CaMK Ⅱα and pCaMK Ⅱα up-regulated, and pCaMK Ⅱα CaMK Ⅱα ratio increased in group DPE ( P 〈 0.05). Condusion Propofol can reduce the cognition impairment induced by ECT in mentally depressed ra

关 键 词:二异丙酚 电惊厥疗法 抑郁 钙-钙调素依赖性蛋白激酶2型 海马 

分 类 号:R965[医药卫生—药理学]

 

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