水稻植株及其内生微生物磷脂脂肪酸生物标记异质性  

Heterogeneity of Phospholipid Fatty Acids Contained in Rice Plant and the Endophytes

作　　者：胡桂萍[1] 刘波[1] 郑雪芳[1] 张建福[1] 谢华安[1] 

机构地区：[1]福建省农业科学院,福建福州350003

出　　处：《热带作物学报》2011年第11期2054-2062,共9页Chinese Journal of Tropical Crops

基　　金：国家863项目"高产优质多抗水稻分子品种创制"(No.2006AA100101);国家科技部973前期项目-芽孢杆菌种质资源多样性及其生态保护功能基础研究(No.2011CB111607);农业部984重点项目"高效新型微生物资源引进与创新"(No.2011-G25);国家863项目"农作物重大病害多功能广谱生防菌剂研究和创制"(No.2006AA10A211);福建省财政专项-福建省农业科学院科技创新团队建设基金(No.STIF-Y03)

摘　　要：采用改良磷脂脂肪酸法分析水稻植物自身磷脂脂肪酸和其内生微生物磷脂脂肪酸生物标记异质性。结果表明,水稻植株和其内生微生物共有磷脂脂肪酸有直链磷脂脂肪酸20∶0、12∶0、16∶0、18∶0和支链磷脂脂肪酸如18∶3ω6с(6,9,12)、19∶0 CYCLOω8с、15∶1ω6с等,且在水稻中含量均低于30μg/g,在内生微生物中的含量高于30μg/g。水稻内生微生物独有磷脂脂肪酸主要为支链磷脂脂肪酸,共有22种如i20∶0、20∶1ω9с、12∶0 3OH等,含量差异很大,为0.91～11 481.25μg/g。对水稻内生微生物独有磷脂脂肪酸对应分析CA得到,遗传距离近的水稻品种其对应距离也近,CA载荷图显示内生微生物磷脂脂肪酸对应距离小于姨2的为多元分布,即该磷脂脂肪酸分布在多个水稻品种中;大于姨2的为单一分布,即仅在特定的水稻品种中分布,表明水稻内生微生物磷脂脂肪酸分布与水稻品种遗传有关。Heterogeneity of phospholipid fatty acids contained in rice plantand their endophyte were analyzed by the modified method of phospholipid fatty acids. The results revealed that phospholipid fatty acids of straight chain phospholipid fatty acid such as 20：0, 12：0, 16：0, 18：0 and unsaturated phospholipid fatty acids such as 18：3ω6c （6, 9, 12）, 19：0 CYCLOω8c, a17：0, i16：1 H, 16：0 N ALCOHOL, 15：1ω6c, i15：0 3OH were found both in rice plants and the endophytes, however, the amounts of the phospholipid fatty acid in rice plant were all less than 30μg/g compared to that of more than 30 μg/g in the rice endophytes. The other 22 phospholipid fatty acids were only detected in the rice endophye, for example i20：0, 20：1 ω9c, 12：0 3OH, the amounts of them were arranged from 0.91-11481.25μg/g. The contents of the phospholipid fatty acids from the endophytes in different rice were different and they represented to the multiple distribution that meant the definite phospholipid fatty acid distributed in the endophytes in more than one rice variety and the single distribution that meant the definite phospholipid fatty acid distributed in the endophytes in only one rice variety. The correspondence analysis of the phospholipid fatty acids from endophytes showed that corresponding distances of the phospholipid fatty acids were related with the heredity in rice varieties. Loading chart of correspondence analysis displayed that phospholipid fatty acids were multiple distribution when their corresponding distances were longer than, contrary to that, they were single distribution. It is shown that the distribution of phospholipid fatty acids of endophytes were relation with the hereditary of rice.

关 键 词：水稻 组培苗 内生微生物 磷脂脂肪酸 异质性 

分 类 号：S511[农业科学—作物学]