植物叶绿体蛋白质组学研究进展  

Research Progress on Plant Chloroplast Proteomics

作　　者：贺庭琪[1] 郭安平[1] 杜伟[1] 常丽丽[1] 王旭初[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2011年第11期2196-2203,共8页Chinese Journal of Tropical Crops

基　　金：国家重点基础研究发展973计划(No.2010CB126605);转基因生物新品种培育科技重大专项(No.2008ZX08012-04);热带生物所基本科研业务费专项(No.ITBB110101;02)

摘　　要：亚细胞蛋白质组学研究是近年来蛋白质组学研究中的一个热点,蛋白质组分析已成为在亚细胞水平鉴定植物功能蛋白的有力工具。叶绿体作为重要的植物细胞器,在植物蛋白质组学中已有较多的研究。随着双向电泳技术的改进和质谱灵敏度的提高,并结合不断增多的拟南芥、水稻、玉米等植物的数据库信息相结合,许多植物叶绿体中的蛋白质已经被成功鉴定。叶绿体蛋白质组为植物亚细胞蛋白质组学研究中相对全面的一部分,利用亚细胞分离结合双向电泳技术来系统地鉴定叶绿体中蛋白质组分是获取叶绿体蛋白质信息、确定其功能的重要技术手段。本文主要介绍了蛋白质组学的发展及蛋白组学研究技术,并对叶绿体各部分蛋白的研究进展进行综述,以全面认识叶绿体蛋白的组成、特点及其在叶绿体代谢网络中的作用。Subcellular proteomics is a hotspot in the study of proteomics in recent years, and proteomic analysis is a powerful approach to identify plant functional proteins. As an important plant organelle, chloroplast has been intensively studied in plant proteomics. With the sensitivity improvement of two-dimensional electrophoresis and mass spectrometry, and combining with the increasing database information from Arabidopsis, rice, corn and other plants, many chloroplast proteins can be quickly identified. Compared with other subcellular proteomics, chloroplast proteome is more comprehensive. Sub -cell separation system, together with two -dimensional electrophoresis that identify the protein components of chloroplast proteins is a useful method to obtain information of chloroplast proteins and to identify the function. This paper mainly describes the development of proteomies and proteomies technologies, and gives a review of chloroplast proteins in order to fully understand the composition and character of chloroplast proteins, and their role in chloroplast metabolic network.

关 键 词：亚细胞蛋白质组学 双向电泳 质谱 生物信息学 叶绿体蛋白 

分 类 号：TQ936.2[化学工程]