妊娠滋养细胞肿瘤外周血肿瘤细胞检测方法的临床研究  

Detection of tumor cells in peripheral blood of patients suffered from gestational trophoblastic

作　　者：朱建春 

机构地区：[1]许昌市中心医院妇产科,河南许昌461000

出　　处：《医药论坛杂志》2011年第21期98-100,共3页Journal of Medical Forum

摘　　要：目的研究用荧光定量逆转录-聚合酶的方法检测妊娠滋养细胞肿瘤患者外周血中肿瘤细胞的方法。方法取正常非妊娠期妇女外周血,将绒癌细胞株(JAR细胞)掺入,用荧光定量逆转录-聚合酶反应检测其中β-hCG-mRNA,推测出JAR细胞的量;同时11例妊娠滋养细胞肿瘤患者,采集治疗前外周血,同样检测其中的β-hCG-mRNA,以推测JAR的含量。结果此检测方法适合一定浓度的JAR,当外周血中含有102以上JAR细胞时可检测到其中的β-hCG-mRNA;11例患者血中有6例的外周血中检测到β-hCG-mRNA表达。根据JAR推算患者的肿瘤细胞量为104～108个/10ml外周血。结论荧光定量RT-PCR法测定B-hCG-mRNA是检测、推算外周血中滋养细胞肿瘤细胞具有一定的效果,可以在临床推广。Objective To found sensitive and reliable method of fluorescence quantitative reverse transcription polymerase chain reaction to identify trophoblastic tumor cells in the peripheral blood of the patients sufered from gestational trophoblastic tumor.Methods Totally 11 case of pregnant peripheral blood as a model.Given numbers of JAR cell from ten to million were mixed into 10ml non-pregnant.Detection of β-hCG-mRNA with fluorescence quantitative reverse transcription polymerase chain reaction（FQ.RT.PCR）and then estimation of the numbers of tumor cell in the blood.Results FQ-RTPCR could detect β-hCG-mRNA,when ≥10 JAR cells were mixed into 10ml non-pregnant peripheral blood.Six cases of bloods had been detected β-hCG-mRNA expression in 11 cases of gestational trophoblastic tumor,and the numbers of tumor cell from 104 to 107 per 10ml blood.Conclusions FQ.RT.PCR is effective method to designed forβ-hCG-mRNA had been proved to be very sensitive detection means,it could be used to detect gestational trophoblastic tumor cells from patient preipheral blood.

关 键 词：绒癌细胞株 Β亚基 MRNA 妊娠滋养细胞肿瘤 逆转录聚合酶链反应 检测 

分 类 号：R737.33[医药卫生—肿瘤]