重组腺病毒载体CRT联合紫杉醇对乳腺癌细胞周期、增殖及凋亡的影响  

Effects of recombinant adenovirus Ad-CRT plus paclitaxol on cell growth,cell cycle and cell apoptosis of breast cancer cell line MDA-MB-231

作　　者：赵丹[1] 刘新莉[1] 仇凤启[1] 孙大鹏[1] 马萍[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所,辽宁沈阳110001

出　　处：《现代肿瘤医学》2012年第1期7-10,共4页Journal of Modern Oncology

基　　金：辽宁省自然科学基金资助项目(No.20042073);辽宁省科技计划项目(No.2009225008-1)

摘　　要：目的:研究Ad-CRT联合紫杉醇对乳腺癌MDA-MB-231细胞周期、细胞增殖及凋亡的影响。方法:MTT法检测细胞增殖,相差显微镜观察细胞形态学变化;PI单染流式细胞术检测细胞周期和凋亡。结果:Ad-CRT联合紫杉醇对乳腺癌细胞MDA-MB-231增殖的抑制率明显高于单纯Ad-CRT组和单纯紫杉醇组,且抑制作用呈时间依赖关系(P<0.05),且细胞形态发生明显的改变。Ad-CRT联合紫杉醇将乳腺癌细胞MDA-MB-231的细胞周期明显的阻滞在G2/M期,并且促进细胞的凋亡。结论:Ad-CRT联合紫杉醇能增强乳腺癌细胞MDA-MB-231增殖抑制、阻滞细胞周期并且诱导细胞凋亡。Objective：To investigate the effects of recombinant adenovirus Ad-CRT plus paclitaxol on cell growth,cell cycle and cell apoptosis of breast cancer cell line MDA-MB-231.Methods： MTT assay was used to detect the effects of Ad-CRT plus paclitaxol on cell growth,cell morphological change was observed by phase contrast microscope.The effects of Ad-CRT plus paclitaxol on cell cycle and cell apoptosis were determined by flow cytometry assay.Results： Ad-CRT plus paclitaxol inhibited the growth of MDA-MB-231 cells more than Ad-CRT and paclitaxol in a time dependent manner（P0.05）.The results of flow cytometry assay showed that Ad-CRT plus paclitaxol inhibited MDA-MB-231 cells on G2/M2 and then stimulated apoptosis.Conclusion： Ad-CRT plus paclitaxol could inhibit the growth of MDA-MB-231 cells,and induce the cycle arrest so as to raise the apoptotic rate.

关 键 词：重组腺病毒载体CRT 紫杉醇 乳腺癌 增殖 凋亡 

分 类 号：R73-36[医药卫生—肿瘤] R737.9[医药卫生—临床医学]