膜型基质金属蛋白酶-1在浸润性肝细胞癌组织的表达及其意义  

Expression of membrane type-1 matrix metalloproteinase in invasive hepatocellular carcinoma

作　　者：徐涛[1] 李乐平[1] 石玉龙[1] 苗瑞政[1] 盛红光[1] 

机构地区：[1]山东大学附属省立医院胃肠外科,250021

出　　处：《中华实验外科杂志》2012年第1期80-82,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨在浸润性肝细胞肝癌（HCC）中，膜型基质金属蛋白酶-1（MT1-MMP）的表达和意义。方法随机选取2004年1月至2006年12月间根治性手术切除的HCC标本123例，通过对肿瘤的个数、包膜是否完整、门静脉有无癌栓、有无肝外转移等标准的划分，将其分为浸润转移组（74）及无浸润组（49）。术前所有病例均采用酶联免疫吸附试验（ELISA）测定血清血管内皮生长因子（VEGF）水平；并通过免疫组织化学法（ABC），测定两组术后标本癌组织的MT1-MMP蛋白及肿瘤微血管密度（MVD）的表达；定量聚合酶链反应（PCR）测定癌组织MT1-MMPmRNA的表达。结果浸润组术前血清VEGF：（133．89±68．56）μg／L；无浸润组：（100．64±81．37）μg/L，差异有统计学意义（P〈0．01）。MT1-MMP主要定位表达在肝癌细胞的胞膜和间质。在浸润组中，MT1-MMP蛋白及mRNA表达量明显高于无浸润组（P〈0．05）；浸润组MVD：11．24±1．49，无浸润组：8．11±2．51，两者比较浸润组MVD的形成量明显增多（P〈0．01）。结论在浸润性肝细胞肝癌中，MT1-MMP的表达明显增高，并伴随血清VEGF的表达上调、癌组织内新生肿瘤微血管的形成增多。MT1-MMP高表达可作为浸润性肝癌的判定指标，并提示临床预后较差。Objective To investigate effect and expression of membrane type-1 matrix metallopro-teinase （MT1-MMP, MMP-14） in invasive hepatocellular carcinoma （HCC）. Methods We selected 123 patients with HCC randomly from January 2004 to December 2006 which underwent radical hepateetomy, they were divided into the invasion group （n = 74） and the non invasion group （n = 49 ） by the standards including tumor size, number, complete capsule, portal vein thrombosis and extrahepatic metastasis. The serum vascular endothelial growth factor （VEGF） was measured with the quantitative sandwich enzyme-linked immunosorbent assay （ELISA） three days before operation. With three-step immunohistochemical technique （ ABC）, to determine protein expression of MT1-MMP and microvessel density （MVD） in post-operative tissue samples in the two groups, and mRNA expression of MT1-MMP was measured by quantitative real-time polymerase chain reaction （PCR）. Results The preoperative serum VEGF in the invasion group （ 133.89±68. 56 ）μg/L was significantly elevated as compared to that in non invasion group （ 100. 64±81.37 ） μg/L （ P 〈 0. 01 ）. Tissue location of MT1-MMP was mainly at membrane and in eyto-plasm of HCC cells. In invasive HCC, mRNA and protein expression of MT1-MMP were significantly increased compared to that in no invasive HCC （P 〈 0. 05 ）, and MVD expression increased accordingly （P 〈 0. 01 ）. Conclusion In invasive HCC, expression of MT1-MMP was significantly increased, and companied with increased secretion of VEGF, also the formation of tumor angiogenesis in HCC increased. High expression of MT1-MMP in postoperative tissue samples could be used as a valuable predictor for invasive HCC, and it prompted poor clinical prognosis.

关 键 词：膜型基质金属蛋白酶-1 血管内皮生长因子 肿瘤微血管密度 转移 预后 

分 类 号：R735.7[医药卫生—肿瘤]