曲古抑菌素A联合单纯疱疹病毒I型对U251细胞增殖、凋亡的影响  

The effects of TSA combined with HSV-1 on proliferation and apoptosis of U251 cells

作　　者：许强华[1] 陈新军[1] 

机构地区：[1]武汉大学中南医院神经外科,430071

出　　处：《中华实验外科杂志》2012年第1期95-96,共2页Chinese Journal of Experimental Surgery

摘　　要：目的以体外培养的U251细胞为模型，观察曲古抑菌素A（TSA）联合单纯疱疹病毒I型（HSV-1）对肿瘤细胞增殖、凋亡的影响。方法以0．5×10^-3mol／LTSA和10MOIHSV-1及0．5×10^-3μmol／LTSA与10MOIHSV-1组合作用于U251人胶质瘤细胞，72h后噻唑蓝（MTr）比色法检测各组细胞增殖情况，显微镜下观察各组细胞形态，流式细胞仪检测各组细胞的凋亡情况。结果单一TSA组、单一HSV-1组、TSA与HSV一1联合组U251细胞增殖抑制率分别为（48．0±1．8）％、（18．0±3．1）％、（58．0±5．8）％；凋亡率分别为（47．4±3．5）％、（29．6±3．0）％、（59．6±4．0）％。TSA与HSV-1联合组U251细胞增殖抑制率及凋亡率明显高于单一使用TSA或HSV-1组（P〈0．01）。结论TSA联合HSV-1对体外培养的U251细胞具有协同或叠加杀伤作用。Objective To observe the effects of trichostain A （TSA） combined with herpes sim- plex virus-1 （HSV-1） on proliferation and apoptosis in U251 cells in vitro. Methods U251 ceils in vitro were treated separately with 0.5×10^-3μmol/Lmol/L TSA （TSA group）, 10 MOI HSV-1 （HSV-1 group）, and 0.5×10^-3μmol/L TSA combined with 10 MOI HSV-1 （TSA ＋ HSV-1 group） for 72 h. The methyl thia- zol tetrazolium （MTT） assay was used to observe the proliferation of U251 cells. The cellular morphological changes were observed under a ruicroscope. The apoptosis rate was analyzed by using flow cytometry. Resuits In TSA group, HSV-1 group, TSA ＋ HSV-1 group, the proliferation inhibition rate of the U251 cells was （48.0±1.8）%, （18.0±3.1）%, and （58.0±5.8）%; Apoptosis rate was （47.4±3.5）%, （29.6±3.0） %, and （ 59. 6±4. 0 ） % respectively. The inhibitory effects of proliferation or apoptosis in TSA± HSV-1 group was stronger than TSA group or HSV-1 group （ P 〈 0. 01 ）. Conclusion TSA combined with HSV-1 has a synergistic killing effect on cultured U251 cells.

关 键 词：曲古抑菌素A 单纯疱疹病毒I型 胶质瘤 增殖 脱噬作用 

分 类 号：R73[医药卫生—肿瘤]