宫颈癌中多药耐药相关蛋白基因表达及意义  

Expression and significance of multi-drug resistance-associated protein gene in cervical cancer

作　　者：高波[1] 杨丰美[2] 余宗涛[1] 张吉才[1] 陶建蜀[3] 

机构地区：[1]湖北医药学院附属太和医院检验部,湖北省十堰市442000 [2]贵州省肿瘤医院妇瘤外科,贵阳市550000 [3]贵阳医学院研究生院,贵阳市550000

出　　处：《中华实用诊断与治疗杂志》2012年第1期41-43,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的探讨多药耐药相关蛋白(multi-drug resistance-associated protein,MRP)基因在宫颈癌组织中的表达情况及临床意义。方法采用RT-PCR技术检测47份宫颈癌组织和12份正常宫颈组织中MRP基因mRNA的表达情况。结果正常宫颈组织中MRP mRNA的表达为0.20±0.11,宫颈癌组织中为0.51±0.28,宫颈癌组织MRP基因表达高于正常宫颈组织(t=6.04,P<0.01)。MRP表达在肿瘤直径、有无淋巴转移、不同肿瘤分化程度、组织学分型及不同临床分期上差异无统计学意义(P>0.05)。结论 MRP基因可能参与宫颈癌原发性耐药的产生,检测其表达对制订治疗方案有一定价值。Objective To investigate the expression and evaluate the clinical significance of multi-drug resistance-associated protein in cervical cancer and normal tissue.Methods The expression of multi-drug resistance-associated protein was detected with reverse transcription polymerase chain reaction in 47 cases of cervical cancer and 12 cases of normal tissue.Results The expression level of multi-drug resistance-associated protein mRNA was significantly higher in cervical cancer tissues（0.51±0.28） than that in normal tissues（0.20±0.11）（t=6.04,P0.01）.The expression of multi-drug resistance-associated protein was not significantly different between patients with and without lymph node metastasis,in patients with different diameters,and with different tumor differentiation degrees and histological types（P0.05）.Conclusion Multi-drug resistance-associated protein mRNA expression may be correlated with intrinsic resistant mechanism in cervical cancer,and the detection of multi-drug resistance-associated protein expression may play a role in making an efficient clinical treatment plan.

关 键 词：宫颈癌 逆转录聚合酶链反应 多药耐药相关蛋白基因 表达 

分 类 号：R737.33[医药卫生—肿瘤]