氡吸入染毒致大鼠相关靶器官的损伤  

Organ damage in rats exposed to radon

作　　者：陈志海[1] 聂继华[1] 武彦文[1] 吴昭昭[1] 张素萍[1] 曹毅[1] 童建[1] 

机构地区：[1]苏州大学医学部公共卫生学院,江苏苏州215123

出　　处：《癌变．畸变．突变》2011年第6期460-464,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81020108028);苏州大学放射医学与公共卫生学院旭日基金

摘　　要：目的:观察氡染毒后大鼠相关靶器官的损伤,特别是肺组织的病理改变,以及肺组织中RAGE和S100A6蛋白的表达,为氡致肺癌的机制研究提供基础资料。方法:健康雄性Wistar大鼠16只,随机分为对照组、低(64 WLM)、中(121 WLM)和高(236 WLM)剂量氡染毒组,每组4只。用多功能生态氡室控制氡浓度在10万Bq/m^3,每天染氡12 h。染毒结束后,观察大鼠肺部病理改变、计算脏器系数、骨髓细胞微核率、检测外周血淋巴细胞的8-羟基脱氧鸟苷(8-OHdG)含量和血清总抗氧化能力(T-AOC);Western blot检测肺组织中RAGE和S100A6蛋白的表达。结果:与对照组比较,高剂量氡染毒组大鼠肺部炎细胞浸润,肺泡间隔增厚,肝脏系数增大,睾丸系数降低,骨髓微核率显著升高,血中8-OHdG含量上升,T-AOC下降;肺组织中RAGE和S100A6蛋白表达上调(P均<0.05)。结论:长期大剂量氡染毒可引起大鼠多器官的损伤,其中肺组织的病变尤为明显。OBJECTIVE：To observe the damage of target organs of rats exposed to radon,especially the pathological changes of lung tissue,as well as the expressions of RAGE and S100A6 proteins,and to provide information for exploring the mechanisms of potential carcinogenic effects of radon on the lungs.METHODS：16 male rats were randomly divided into 4 groups,i.e.control group,low（64WLM）、middle（121 WLM） and high（236 WLM） dose groups,with 4 rats in each group.The Wistar rats were exposed to radon in a multifunction ecological radon room at a constant level of 100 000 Bq/m,12 h each day.Pulmonary pathological changes were examined,then the organ coefficients and the rate of bone marrow micronucleus were calculated,8-OHdG in peripheral blood lymphocytes was assessed by ELISA and T-AOC in serum was determined by ultraviolet spectrophotometer.The RAGE and S100A6 proteins were measured by western blot.RESULTS：In the high dose group,inflammatory cell infiltration and thickening of alveolus septa were observed.The liver coefficient increased and the testis coefficient decreased.Rate of bone marrow micronucleus significantly increased.Level of 8-OHdG in peripheral blood lymphocytes was elevated and that of T-AOC declined.The expressions of RAGE and S100A6 in lung tissue were up-regulated in a dose-response relationship. CONCLUSION：Chronic exposure to radon may induce multi-organ damage,especially pathological alterations of lung tissue at high exposure dose.

关 键 词：氡 RAGE S100A6 8-羟基脱氧鸟苷 总抗氧化能力 

分 类 号：R734.2[医药卫生—肿瘤]