火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究  

Effects of hemp seed oil on learning and memory performances in senile mice induced by D-galactose

作　　者：苏婧[1] 贺海波[2] 石孟琼[2] 熊兴军 陈良金[2] 卢训丛[2] 

机构地区：[1]随州市中心医院药剂科 [2]三峡大学医学院 [3]宜昌市长阳县中医院

出　　处：《中国临床药理学与治疗学》2011年第12期1332-1339,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究火麻仁油对D-半乳糖(D-gal)致衰老小鼠学习记忆能力的保护作用,并探讨其作用机制。方法:采用D-半乳糖诱导小鼠衰老模型,用跳台法、水迷宫法和Morris水迷宫法测定衰老小鼠学习记忆能力,检测小鼠脑组织丙二醛(MDA)含量和总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及大脑皮层和海马组织中乙酰胆碱(Ach)含量和乙酰胆碱酯酶(AchE)、胆碱乙酰化转移酶(ChAT)活性,观察脑组织组织形态学变化。结果:火麻仁油(3、6和12mL/kg)能明显延长跳台实验的跳台潜伏期,缩短错误次数;缩短水迷宫实验达到时间和减少错误次数;缩短Mor-ris水迷宫实验逃避潜伏期和第1次到达原平台时间,增加穿越原平台次数和逗留时间;能显著增加脑组织中T-AOC、SOD、GSH-Px活性和降低MDA含量,增加大脑皮层和海马组织中Ach含量和AchE、ChAT活性,减轻脑组织损伤。结论:火麻仁油可显著改善D-半乳糖致衰老小鼠学习记忆能力,其机制可能与其提高脑组织抗氧化和清除自由基能力,增强中枢胆碱能神经系统功能有关。To research the effects of hemp seed oil on learning and memory performances in senile mice induced by Dgalactose and its mechanisms. METHODS： By using the Dgalactoseinduced aging mice, learning and memory performances were tested with step down, water maze and Morris water maze test, and de termined the activities of total antioxidant capac ity （T AOC）, superoxide dismutase （ SOD）, glutathione peroxidase （GSH-Px） and content of malonaldehyde （MDA） in brain tissue, determined the content of acetylcholine （Ach） and ac- tivities of acetylcholine esterase （AchE）, choline acetyltransferase （CHAT） in cerebral cortex and hippocampus; analyzed brain tissue histopathological examination simultaneously. RESULTS： Hemp seed oil （3, 6, 12 mL/kg） could signifi cantly prolong the latency, reduce the error frequency in step down test, reduce the reaching time and error frequency in water maze test, ohviously shorten the escape latency and reaching primary platform time, increase passing through primary platform frequency and resided time in Morris water maze test. It could obviously re- duce the content of MDA, increase activities of T-AOC, SOD, GSH-Px in brain tissue, remarkably increase the content of Ach and activities of AchE, ChAT in cerebral cortex and hippocam pus and reduce the brain tissue injury. CONCLUSION： Hemp seed oil improves the learning and memory performances of the Dgalactoseinduced aging mice. The mechanism may be related to increased activity of antioxidation and scav enge of free radicals, improved function of the central eholinesterase svstom

关 键 词：火麻仁油 衰老 学习记忆 活性氧基团 胆碱能神经系统 小鼠 

分 类 号：R-33[医药卫生]