溃疡性结肠炎模型的建立及影响因素  

Establishment of a rat model of ulcerative colitis and analysis of factors influencing the development of this model

作　　者：侯丽娟[1] 唐方[1] 王晓红[1] 孙晓萍[1] 

机构地区：[1]天津医科大学总医院中医科,天津市300052

出　　处：《世界华人消化杂志》2011年第31期3242-3245,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨TNBS/乙醇法制备溃疡性结肠炎模型时乙醇最佳浓度及最宜动物室环境.方法:Wistar大鼠45只,随机分为正常组、模型1组、模型2组、模型3组、模型4组(9只/组),其中将正常组、模型1、2、3组放入清洁环境饲养,模型4组放入SPF级动物实验室饲养.分别用9g/LNaCL、TNBS/无水乙醇、TNBS/600mL/L乙醇、TNBS/500mL/L乙醇灌肠,其中模型3、4组均用TNBS/500mL/L乙醇灌肠,造模后观察各组模型疾病活动指数,实验终点期处死后观察结肠黏膜损伤指数及病理组织学改变.结果:模型1、2组灌肠后大量死亡,无实践意义;将模型3、4组DAI及CMDI评分比较,模型3组均高于模型4组,且差异具有显著性(P<0.05).组织学观察比较模型3、4组,具有明显差异.结论:应用TNBS建立UC模型应首选500mL/L乙醇作为溶剂,并需在清洁级动物室进行,环境是提高模型成功率的关键因素.AIM：To investigate the optimal concentration of ethanol and animal house environment for the development of a rat model of trinitrobenzenesulfonic acid（TNBS） /ethanol-induced ulcerative colitis.METHODS：Forty-five Wistar rats were divided randomly and equally into five groups：normal group,model groups 1,2,3 and 4.The normal group,model groups 1,2 and 3 were fed in conventional grade animal house,while the model group 4 was fed in SPF grade animal house.The normal group,model groups 1,2,3 and 4 were given 9 g/L NaCL,TNBS/ethanol,TNBS/600 mL/L ethanol,TNBS/500 mL/L ethanol,and TNBS/500 mL/L ethanol,respectively.After treatment,disease activity index（DAI） was monitored.At the end of the experiment,all rats were killed to evaluate colon mucosa damage index（CMDI） and histological changes.RESULTS：There was no significant difference inmortality between the model groups 1 and 2.DAI and CMDI significantly increased in the model group 3 compared to the model group 4（both P 0.05） .Histological changes also differed significantly between the model groups 3 and 4. CONCLUSION：The development of a rat model of TNBS-induced ulcerative colitis should be done with 500 mL/L ethanol in conventional grade animal house.

关 键 词：三硝基苯磺酸 溃疡性结肠炎 模型 乙醇浓度 

分 类 号：R574.62[医药卫生—消化系统]