RUNX3基因在宫颈癌中的表达及临床意义  

Expression of RUNX3in cervical carcinoma and its clinical significance

作　　者：邓玉屏[1] 聂妹芳[1] 刘凤英[1] 蒋姗[1] 刘毅智[1] 李登清[2] 孟渠成[3] 李津[4] 黄民主[5] 汪明明[1] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011 [2]中南大学湘雅医学院检验系,长沙410013 [3]中南大学湘雅医学院科研办,长沙410078 [4]中南大学湘雅三医院麻醉科,长沙410013 [5]中南大学公共卫生学院流行病学教研室,长沙410078

出　　处：《中南大学学报（医学版）》2011年第12期1189-1194,共6页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅项目(2007.sk3043)~~

摘　　要：目的:探讨RUNX3在宫颈癌发生、发展中的意义。方法:采用免疫组织化学方法检测25例正常宫颈、34例宫颈上皮内瘤变(intraepithelia neoplasia,CIN)、48例宫颈癌组织中RUNX3蛋白的表达,应用SYBR GreenⅠ嵌合荧光real-time PCR技术检测10例正常宫颈、24例CIN和30例宫颈癌组织中RUNX3mRNA的表达。结果:RUNX3蛋白、mRNA在正常宫颈、CINⅠ、CINⅡ～Ⅲ和宫颈癌中的表达呈递减趋势,4组间差异有统计学意义(P<0.05)。RUNX3蛋白、mRNA的表达与宫颈癌分化程度、临床分期以及是否淋巴结转移明显相关(P<0.05),与年龄、组织学类型和高危型人乳头状瘤病毒感染无显著相关(P>0.05)。结论:RUNX3可能作为抑癌基因参与了宫颈癌的发生、发展。Objective To explore the role of runt-related transcription factor 3（RUNX3） in the tumorgenesis and progression of cervical carcinoma.Methods The immunohistochemical staining technique was used to detect the expression of RUNX3 protein in 25 cases of normal cervix,34 intraepithelia neoplasia（CIN）,and 48 cervical carcinomas.SYBR GreenⅠchimeric fluorescence Real-time PCR was applied to detect the expression of RUNX3 mRNA in 10 cases of normal cervix,24 CIN,and 30 cervical carcinomas.Results The expressions of RUNX3 protein and mRNA in normal cervix,CINⅠ,CINⅡ-Ⅲ,and cervical carcinoma tissues tended to be down-regulated.There was significant difference among these groups（P0.05）.The expressions of RUNX3 protein and mRNA in the cervical carcinoma tissues were correlated with the histological differentiation,clinical stage,and lymphatic metastasis（P0.05）,but had no relationship with the age,high-risk human papillomavirus infection,and histological classification（P0.05）.Conclusion RUNX3 may function as a tumor suppressor gene in the occurrence and progression of cervical carcinoma.

关 键 词：宫颈癌 宫颈上皮内瘤变 RUNX3 免疫组织化学 实时定量PCR 

分 类 号：R737.33[医药卫生—肿瘤]