胰腺癌中Toll样受体2和血管内皮生长因子的表达及临床意义  

Expression and clinical significance of TLR2 and VEGF in pancreatic cancer

作　　者：蒯勇[1] 孟令新[2] 郭冬[2] 

机构地区：[1]山东省日照市人民医院肝胆外科,276826 [2]山东省日照市人民医院肿瘤科,276826

出　　处：《中国实用医刊》2012年第2期4-8,共5页Chinese Journal of Practical Medicine

基　　金：山东省自然科学基金资助项（ZR2010HL053）

摘　　要：目的探讨Toll样受体2（TLR2）和血管内皮生长因子（VEGF）在胰腺癌中的表达及其临床意义。方法采用实时荧光定量PCR检测40例新鲜胰腺癌及相应癌旁组织标本中TLR2及VEGFmRNA的水平；应用免疫组化检测TLR2和VEGF蛋白在60例胰腺癌及相应癌旁组织中的表达，并分析二者与临床病理学特征之间的关系。应用Kaplan-Meier法分析TLR2和VEGF蛋白表达对患者生存期的影响。结果胰腺癌组织中的TLR2和VEGFmRNA相对水平分别为0．75±0．16和0．82±0．21，显著高于癌旁组织的0．36±0．12和0．38±0．15（P均〈0．05）；胰腺癌中TLR2和VEGF蛋白表达阳性率分别为56．67％和68．33％，显著高于癌旁组织的31．67％和36．67％（P均〈0．05）。TLR2、VEGF蛋白表达与患者性别、年龄、肿瘤部位及分化程度无相关性，而与肿瘤大小、淋巴结转移、神经转移及临床分期相关。TLR2、VEGF阴性组患者生存期分别为16．2个月和19．5个月，显著长于TLR2、VEGF阳性组（分别为10．4个月和12．6个月，P均〈0．05）。结论TLR2、VEGF介导了胰腺癌的发生、发展，与临床病理学特征关系密切。Objective To study the expression of Toll-like receptor 2 （TLR2） and VEGF in pan- creatic cancer and its clinical significance. Methods The levels of TLR2 and VEGF mRNA were detec- ted in 40 cases of pancreatic cancer and corresponding adjacent tissues by real-time fluorescence quantita- tive PCR. The expression of TLR2 and VEGF proteins were detected in 60 cases of pancreatic cancer and corresponding adjacent tissues by immunohistochemistry. Relationship between TLR2 and VEGF with clinieopathological characteristics was analyzed. Kaplan-Meier analysis was used to analyze effect of TLR2 and VEGF protein expression on the survival of patients. Results TLR2 and VEGF mRNA were 0. 75 ±0. 16 and 0. 82 ±0. 21 in pancreatic cancer tissue, significantly higher than that in the adjacent tissue[ （0.36 ±0. 12） and （0.38 ±0. 15） ] （ P all 〈0.05）. TLR2 and VEGF protein positive rate was 56.67% and 68.33% in pancreatic cancer tissue, significantly higher than that in the adjacent tissue （31.67% and 36.67% ） （ P all 〈0. 05 ）. TLR2, VEGF protein expression has no rebationship with pa- tient gender, age, tumor location, and the degree of differentiation, but has relationship with tumor size, lymph node, nerve metastasis and clinical staging. TLR2, VEGF-negative patients＇ survival time was 16. 2 months and 19. 5 months, significantly longer than TLR2, VEGF-positive group（ 10. 4 months and 12. 6 months, P all 〈 0. 05 ）. Conclusions TLR2, VEGF mediates the development of pancreatic canc- er, and were closely related with clinical pathological features.

关 键 词：TOLL样受体2 血管内皮生长因子 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]