补肾填精方对Aβ_(1-40)所致老年性痴呆模型大鼠行为学及病理学的影响  

Effects of Nourishing Kidney-essence Formula on Behavior And Pathological Changes in Aβ_(1-40) Induced Senile Dementia Rats

作　　者：安红梅[1] 张占鹏[1] 史云峰[1] 陈久林[2] 顾明昌[1] 

机构地区：[1]上海中医药大学附属龙华医院脑病科,上海200032 [2]上海市中医老年医学研究所,上海200031

出　　处：《中华中医药学刊》2012年第1期23-26,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81072789);国家基础研究发展计划(973计划)资助项目(2010CB530400);上海市教委创新项目(10YZ58);上海市科委中药现代化项目(10DZ1971200)

摘　　要：目的:观察补肾填精方对Aβ1-40所致老年性痴呆模型大鼠行为学及病理学影响。方法:84只SD大鼠随机分为空白组,模型组,补肾填精方小剂量组、中剂量组、大剂量组,双益平组。采用大鼠双侧海马CA1区注射Aβ1-40复制老年性痴呆模型。造模24h后开始连续给药3周。Morris水迷宫法测试大鼠学习记忆能力,透射射电镜观察大鼠海马CA1区神经元、突触的变化。结果:补肾填精方可改善大鼠的学习记忆能力、改善海马CA1区神经元结构、减少突触的丧失。结论:补肾填精方能改善Aβ1-40所致老年性痴呆模型大鼠行为学及脑组织的病理损害。Objective： To observe the effects of nourishing kidney-essence formula on the behavior and pathological changes in senile dementia rats induced by Aβ1-40.Methods：84 SD rats were randomly divided into normal group,model group,low-dose,middle dose and high-dose of nourishing kidney-essence formula group,and Huperzine A group.Senile dementia was induced by Aβ1-40 injection in bilateral hippocampal CA1 area of these rats.After 24h of Aβ1-40 injection,drugs were continuously given for 3 weeks.Learning and remembering abilities were observed by water maze test,neurons and synaptic changes in hippocampal CA1 were observed by transmission electronic microscopy.Results： Learning and remembering abilities of senile dementia rats were significantly improved by nourishing kidney-essence formula treatment,especially in middle dose of nourishing kidney-essence formula.Nourishing kidney-essence formula also showed the effects of improving structure of hippocampal CA1 neurons,and reducing synapse loss.Conclusion： Nourishing kidney-essence formula could improve the behavior and pathological damage in hippocampal CA1 in Aβ1-40 induced senile dementia Rats.

关 键 词：中医药 补肾填精方 老年性痴呆 学习记忆 海马神经元 

分 类 号：R749.16[医药卫生—神经病学与精神病学]