槲寄生碱体外通过激活Caspase8诱导ACC细胞的凋亡  

Inhibitory effects of mistletoe alkali on the ACC cells by active Caspase-3

作　　者：邱心如[1] 周洪澜[2] 李亚娟[3] 张丽红[4] 翟颖仙[4] 王医术[4] 

机构地区：[1]吉林大学药学院,2009级吉林长春130021 [2]吉林大学第一医院,吉林长春130021 [3]四平市中心人民医院 [4]吉林大学病理生物学教育部重点实验室,吉林长春130021

出　　处：《中国实验诊断学》2012年第1期27-28,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划(201015198);吉林省杰出青年资助项目(20070132);大学生创新计划(2010A73113)

摘　　要：目的探讨槲寄生碱(mistletoe alkali,MA)通过激活Caspase8途径诱导涎腺腺样囊性癌(ACC)细胞凋亡的研究。方法以5-氟尿嘧啶(5-FU)作用组为阳性对照组,以不加药组为阴性对照组,槲寄生碱处理组为实验组作用ACC细胞,采用免疫组织化学染色方法,检测三组细胞中Caspase8蛋白的表达情况。结果 槲寄生碱组Caspase8表达明显高于对照组(P<0.01),并且随着槲寄生碱浓度越高,Caspase8表达越强。结论槲寄生碱可以通过激活Caspase 8途径来诱导ACC细胞凋亡,并呈剂量依赖性。Objective to study whether alkaloid from mistletoe induces apoptosis of ceils of adenoid cystic carcinoma of salivary gland （ACC cell） via activation of caspase8;methods The ACC cells treated by 5-FU were used as positive control group and the ACC cells without any treatment as negative control group, the ACC cells treated by mistletoe alkali were used as experimental groups, the expression of alkaloid groups is obviously higher than the control groups, Results The expression of caspase8 of alkaloid group was much higher than that of control groups（P〈0.01） ,and the expression is stronger with the higher concentration of alkaloid. Conclusion alkaloid of mistletoe induces apoptosis of ACC cell via activation of caspase8 and the increased easpase8activity is dose dependent.

关 键 词：槲寄生碱 CASPASE 8 ACC细胞 凋亡 

分 类 号：R285[医药卫生—中药学]