适合虫草素积累的蛹虫草液体培养条件的优化  

Optimization of Liquid Surging Fermentation Conditions with Cordyceps militaris for Producing Cordycepin

作　　者：欧阳召[1] 王春梅[1] 陶志[1] 

机构地区：[1]北京中医药大学中药学院生物制药系,北京100102

出　　处：《中国食用菌》2012年第1期26-28,共3页Edible Fungi of China

基　　金：北京中医药大学自主选题项目"热敏长循环虫草素脂质体的制备工艺研究"

摘　　要：为了探索蛹虫草液体发酵中适合虫草素积累的培养基和发酵培养条件,以半定量薄层色谱法为检测手段,以虫草素含量为指标,通过单因素试验及正交试验优选的方法,最终优选出的培养基配方为蛋白胨15 g.L-1、葡萄糖20 g.L-1、磷酸二氢钾1 g.L-1、七水合硫酸镁0.5 g.L-1、维生素B1 10 mg.L-1、萘乙酸2 mg.L-1。优选出的培养条件为发酵液pH7,培养温度25℃,光照时间12 h。To find out the best Cordyceps militaris fermentation medium and growth conditions for producing cordycepin, the composition of the culture medium, the culture temperature and light time were investigated by single factor experiment and orthogonal test. Half quantitative thin layer chromatography method was used to measure the concentration of the produced cordycepin. The results showed that the optimal culture medium were peptone 15 g·L-1, glucose 20 g·L-1, KH2PO4 1 g·L-1, MgSO4·H2O 0.5 g·L-1, VB1 10 mg·L-1, 1-naphthlcetic acid 2 mg·L-1 and pH7. The best temperature was 25~C and the light length was 12 h per day.

关 键 词：蛹虫草 虫草素 培养基 培养温度 光照 

分 类 号：S567.35[农业科学—中草药栽培]