一种微藻多糖的理化性质及抗氧化和保湿活性  

Physicochemical Property Analysis of Polysaccharides from Marine Microalgae and Their Antioxidation,Hygroscopicity and Moisture Retention Activities

作　　者：王凌[1] 孙利芹[1] 赵小惠[1] 

机构地区：[1]烟台大学生物工程系,山东烟台264005

出　　处：《精细化工》2012年第1期20-24,29,共6页Fine Chemicals

基　　金：国家"十一五"科技支撑计划项目(2008BAD94B04);山东省自然科学基金项目(Y2007D59Y2008B21);烟台市科技发展计划项目(2009219)~~

摘　　要：采用超声辅助H2O2-Vc体系降解Sarcinochrysis marina Geitler多糖(SMP-0),经Sepherose 6B分级纯化得到3种相对均一的多糖组分(SMP-1、SMP-2、SMP-3)。分析各组分多糖的理化性质,并通过总还原力、自由基清除及吸湿、保湿率的测定研究其抗氧化和保湿活性。结果表明:SMP-0、SMP-1、SMP-2和SMP-3是硫酸酯多糖,均由阿拉伯糖、D-果糖和葡萄糖组成,但其组成的摩尔比有差异;抗氧化能力与相对分子质量(简称分子量,下同)大小成反比,且呈剂量依赖。SMP-3的总还原力与Vc相近,对O2-.和.OH的最大清除率分别为94.89%和88.98%;高分子量多糖的吸湿和保湿活性优于低分子量多糖,SMP-0最大吸湿率为49.18%,比甘油小10.5%,比透明质酸和壳聚糖分别大27.4%和35.2%;SMP-0的保湿率为77.23%,明显好于甘油(30.02%),但略弱于透明质酸和壳聚糖。Sarcinochrysis marina Geitler polysacchafide （ SMP - 0） was degraded by H2 02 - Vc solution system. After separation and purification by Sepherose 6B, three kinds of relatively homogeneous products were obtained （SMP - 1, SMP - 2, SMP - 3 ）. The physicochemical properties of the different polysaccharides were analyzed and the antioxidation, hygroscopicity and moisture retention activities were evaluated by determining the total reducing capacity, the scavenging ability of free radicals and moisture retention ratio. The results show that SMP - 0, SMP - 1, SMP - 2 and SMP - 3 were sulfated polysaccharides and consisted of arabinose, D-fructose and glucose, but they differed from each other in molar ratios. The antioxidant abilities were in inverse proportion to molecular weight of polysaccharides, and exhibited dose-dependence. The total reducing capacity of SMP - 3 was similar to that of vitamin C. The maximum scavenging activity of SMP - 3 on O2 ·and· OH were 94.89% and 88.98% ,~＇espectively. The hygroscopicity and moisture retention activity of high molecular weight （ Mw） polysaccharide was superior to that of low Mw fragments. The maximum hygroscopic ratio of SMP -0 was 49. 18%, which was 10.5% lower than that of glycerol, but was 27.4% and 35.2% higher than that of hyaluronic acid and chitosan, respectively. The maximum moisture retention ratio of SMP -0 was 77.23%, which was obviously higher than that of glycerol （30.02%）, but was appreciably lower than that of hyaluronic acid and chitosan.

关 键 词：微藻 多糖 抗氧化活性 吸湿性 保湿性 生物工程 

分 类 号：TQ464.1[化学工程—制药化工]