贝壳多孔羟基磷灰石基骨修复材料和骨形成蛋白-2联合应用引导犬牙周组织再生效果初步评估  

Core-shell Hydroxyapatite Combined with Bone Morphogenetic Protein 2 in Periodontal Regeneration Treatment in Dogs

作　　者：陈斌[1] 吴文蕾[1] 孙卫斌[1] 韩方凯[2] 张其清[3,4] 

机构地区：[1]南京大学医学院附属口腔医院牙周科,江苏南京210008 [2]南京大学医学院附属口腔医院影像科 [3]厦门大学生物医学工程研究中心 [4]天津市生物医学材料重点实验室

出　　处：《广东牙病防治》2011年第12期619-623,共5页Journal of Dental Prevention and Treatment

基　　金：南京市医学科学技术发展专项项目(ZKX07022);天津市生物医学材料重点实验室开放课题;南京市医学科技发展青年人才启动项目(QYK10166)

摘　　要：目的初步评估贝壳多孔羟基磷灰石基骨修复材料及该材料和骨形成蛋白-2联合应用引导比格犬牙周组织再生的效果。方法选取18月龄比格犬6只,牙周基础治疗后1周,在下颌第二、三、四前磨牙,建立急性牙周骨缺损模型,依照分组情况进行不同治疗。实验组(T组)植入骨修复材料和骨形成蛋白-2;阴性对照组(NC组)植入骨修复材料;空白对照组(BC组)不植入任何材料。实验设计采取同颌同名牙对照,同一只比格犬的3对同颌同名牙分别为:空白对照组和阴性对照组,阴性对照组和实验组,空白对照组和实验组。术后12周,处死动物,Micro-CT检查并对数据进行统计学分析。结果材料植入后,未见材料溢出,植入局部和全身都未见明显不良反应。3组缺损都有一定程度骨再生,以T组再生组织量最多,BC组最少。Micro-CT结果显示:T组、NC组和BC组的骨再生平均高度为(4.50±0.47)mm(、1.75±0.42)mm和(0.87±0.31)mm。NC组和BC组相比,差异有统计学意义(P<0.05)。T组与NC组和BC组相比,差异均有统计学意义(P<0.05),且有临床意义。结论贝壳多孔羟基磷灰石基骨修复材料和骨形成蛋白-2联合应用于比格犬,可以获得更好的引导组织再生效果。Objective To evaluate the ability of core-shell hydroxyapatite bone graft material alone and combined with bone morphogenetic protein 2（BMP-2） in periodontal regeneration treatment in dogs.Methods Thirty-six defects were created in six 18-months male beagle dogs at the sites of the second,third and fourth mandibular premolars one week later after the dogs were treated with non-surgical periodontal therapy.Different treatments were carried out according to which group the teeth belonged.There are 3 groups.The test group（group T） was treated with core-shell hydroxyapatite bone graft material combined with BMP-2;the negative control group（group NC） was treated with core-shell hydroxyapatite bone graft material alone and the blank control group（group BC） was treated with no graft material.When a tooth was selected into one of the 3 groups randomly,the tooth with the same name on the other side in the same jaw was selected into another group.6 defects in the same dog were made and divided into 3 groups.The animals were sacrificed 12 weeks after surgery and analyzed by Micro-CT.Results There was no adverse reaction after material was planted.There were bone regenerations in all groups.Group T got the best bone regeneration while group BC got the least bone regeneration.The biggest height of bone regeneration for group T,group NC and group BC were（4.50±0.47） mm、（1.75±（0.42） mm） and（0.87±0.31） mm according to Micro-CT evaluation.There were statistical difference of bone regeneration height between group NC and group BC.There were statistical differences between group T and group NC and between group T and group BC.Conclusion More periodontal tissue regeneration could be got when core-shell hydroxyapatite bone graft material was used with bone morphology protein 2.

关 键 词：牙周组织再生 贝壳多孔羟基磷灰石 骨形成蛋白-2 牙周治疗 

分 类 号：R781.4[医药卫生—口腔医学]