机械应力刺激对成牙骨质细胞骨桥蛋白表达影响的研究  

Effects of Mechanical Strain on Expression of OPN mRNA in Cementoblasts in Vitro

作　　者：包丽娜[1] 段培佳[1] 任柏林[2] 柴巧学[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院,广东广州510280 [2]贵阳医学院

出　　处：《广东牙病防治》2011年第12期624-627,共4页Journal of Dental Prevention and Treatment

基　　金：广东省医学科研基金项目(A2008105)

摘　　要：目的研究机械应力刺激对成牙骨质细胞矿化相关基因骨桥蛋白(osteopontin,OPN)mRNA表达的影响。方法本研究设3个实验组和1个对照组,利用四点弯曲细胞力学加载装置,对体外培养的成牙骨质样细胞OCCM-30施加2 000μstrain张应力2、000μstrain压应力和4 000μstrain压应力,对照组不加力。分析加力3 h、6 h、12 h、24 h时成牙骨质样细胞OCCM-30 OPN mRNA表达差异,对数据进行统计学分析。结果和对照组相比,2 000μstrain压应力组和4 000μstrain压应力组在加力初期(3 h)OPN的mRNA表达增加,加力后期(24 h)其mRNA表达受到抑制,差异有统计学意义(P<0.05);2 000μstrain张应力组在加力3 h、6 h和对照组相比,OPN mRNA的表达差异无统计学意义(P>0.05),加力12 h、24 h OPN mRNA的表达受到抑制,差异有统计学意义(P<0.05)。结论机械应力刺激可以调节成牙骨质细胞OPN mRNA的表达,可能由此影响牙根吸收和修复过程。Objective To evaluate the effects of the mechanical strain on the expression of the mineral associated gene OPN in cementoblast in vitro.Methods Cementoblasts OCCM-30 were subjected to mechanical strain by four-point bending system with 2 000 μstrain tension,2 000 μstrain compression and 4 000 μstrain compression for 3 h,6 h,12 h,24 h.The expression of OPN mRNA were analyzed with real time quantitive RT-PCR after mechanical loading.ResultsCompare with controls,2 000 μstrain and 4 000 μstrain compression enhanced OPN mRNA expression at 3 h,and suppressed at 24 h.There was a significant difference between them（P0.05）.2 000 μstrain tension had no statistical significant at 3,6 h（P0.05） and had statistical significance at 12,24 h（P0.05）.Conclusion Mechanical stress can affect the OPN mRNA expression of cenmentblast,and may play a role in regulating orthodontic-related root resorption/repair.

关 键 词：机械应力 成牙骨质细胞 骨桥蛋白 牙根吸收 牙根修复 

分 类 号：R781.4[医药卫生—口腔医学]