腺病毒介导的神经营养素-3基因能够在大鼠脊髓前角运动神经元内过表达神经营养素-3  

Adenovirus-mediated neurotrophin-3 gene can over-express neurotrophin-3 in the motoneurons located at ventral horn of rat spinal cord

作　　者：陈元峰[1] 曾湘[1] 曾园山[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室,广东广州510080

出　　处：《解剖学研究》2011年第6期405-408,共4页Anatomy Research

基　　金：国家自然科学基金(30270700);广东省社会发展攻关基金(2003C33808)

摘　　要：目的观察腺病毒介导的神经营养素-3(NT-3)基因在发出坐骨神经传出纤维的大鼠脊髓前角运动神经元的过表达。方法在坐骨神经内直接注射含有绿色荧光蛋白(GFP)基因(报告基因)的NT-3基因重组腺病毒(Ad-NT-3-GFP),7d后应用免疫荧光组织化学染色技术,在荧光显微镜下观察脊髓前角运动神经元的NT-3过表达。结果 GFP表达组(对照组)和NT-3加GFP表达组两组动物的L4和L5脊髓段横切片上,有绿色荧光蛋白阳性标记的细胞。在NT-3加GFP表达组,还可以观察到NT-3阳性标记的细胞,这种细胞能与绿色荧光蛋白阳性标记的细胞重合,是过表达NT-3的前角运动神经元。与GFP表达组的前角运动神经元形态比较,NT-3加GFP表达组的过表达NT-3的前角运动神经元呈现更富有分支的突起。结论腺病毒介导的NT-3基因能够在发出坐骨神经传出纤维的大鼠脊髓前角运动神经元内过表达NT-3,这为下一歩应用NT-3基因治疗策略修复实验性脊髓损伤提供初歩的实验资料。Objective To observe whether adenovirus-mediated neurotrophin-3（NT-3） gene could over-express neurotrophin-3 in the motoneurons located at ventral horn of rat spinal cord,and derived efferent fibers of sciatic nerve.Methods NT-3 gene recombination adenovirus with green fluorescent protein（GFP） gene（report gene） were injected into the sciatic nerve.NT-3 overexpression of motoneurons located at ventral horn of spinal cord were observed under the fluorescent microscope,using immunofluorescence histochemistal staining technique,seven days after injecting the gene recombination adenovirus.Results GFP positive labeling cells were observed on cross sections of L4 and L5 spinal cord segments in the animals of the GFP express（the control group） and NT-3＋GFP express groups.In the NT-3＋GFP express group,NT-3 positive labeling cells were observed also in L4 and L5 spinal cord segments.These cells were merged with GFP positive labeling cells,and were ventral horn＇s motoneurons overexpressing NT-3.Compared with the morphous of ventral horn＇s motoneurons in the GFP express group,motoneurons overexpressing NT-3 showed more branching processes in the NT-3＋GFP express group.Conclusion Adenovirus-mediated NT-3 gene can over-express neurotrophin-3 in the motoneurons located at ventral horn of rat spinal cord and derived efferent fibers of sciatic nerve.The finding provides an initial experimental data for utilizing further a strategy of NT-3 gene therapy to repair experimental spinal cord injury.

关 键 词：神经营养素-3 腺病毒 坐骨神经 脊髓 运动神经元 基因治疗 

分 类 号：R744[医药卫生—神经病学与精神病学]