30日龄Fmr1基因敲除小鼠的避暗实验观察  

Behavioural comparision of Fmr1 knockout mice at 30 days Age in step-through test

作　　者：黄月玲[1] 黄庆辉[2] 方敏华[3] 孙卫文[3] 李敏雄[3] 戴丽军[1] 陈盛强[1] 

机构地区：[1]广州医学院实验动物中心,广东广州510182 [2]广州医学院第一附属医院,广州510340 [3]广州医学院第二附属医院,广州510260

出　　处：《解剖学研究》2011年第6期427-429,433,共4页Anatomy Research

基　　金：广东省自然科学基金项目(815101700100005);广东省科技计划项目(2005B60302004;2008B030301371;2009B030801368;2009B060300018);广东省中医药管理局建设中医药强省科研基金项目(2008184);广州医学院科研立项项目(04-k-53);广州市中医药中西医结合科研立项项目(2008A52)

摘　　要：目的实验对30日龄的Fmr1基因敲除小鼠的避暗实验进行观察。方法采用30 d龄的KO鼠和WT鼠分别连续进行2 d的避暗实验,根据所获得的数据进行多因素方差分析处理。结果 KO鼠的电击次数与WT鼠相比显著增多,具有统计学意义P<0.05,潜伏期只有第2天雄性相比显著(P<0.05),其他均无明显差异;同周龄KO鼠潜伏期比WT鼠明显少(P<0.05);而KO鼠的错误次数比WT鼠明显多(P<0.05);不同周龄KO鼠或WT鼠的潜伏期、错误次数无差异(P>0.05);第1天KO鼠的潜伏期和错误次数与第2天相比无差异(P>0.05);第1天WT鼠的潜伏期和错误与第2天相比有差异(P<0.05)。结论 30日龄Fmr1基因敲除小鼠的认知能力相对低下。Objective To compare the behaviour defferences at 30 days age in step-through test.Methods Fmr1 knockout mice were identified using the PCR technica,and open field were used in the study.The data was analyzed with Multifactor Variance Analysis.Results KO mice obviously have the shorter latency than the WT mice of the same week,but KO mice obviously have more number of errors than WT mice（P0.05）;On the first day,the latency and number of errors of KO mice have no difference compared with the second day（P0.05）;On the first day,the latency and number of errors of WT mice have distinct difference compared with the second day（P0.05）.Conclusion Fmr1 knockout mice displayed cognitive impairment in step-through test at 30 days age.

关 键 词：避暗实验 FMR1基因敲除 小鼠 脆性X综合症 

分 类 号：R-332[医药卫生]