某种正压生物防护服的病毒气溶胶防护性能评价  

Evaluation of the protective performance of a positive pressure bio-protective clothing against viral aerosol

作　　者：李娜[1] 温占波[1] 杨文慧[1] 王洁[1] 李劲松[1] 胡凌飞[1] 董晓凯[1] 刘克洋[1] 曹杰[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室国家生物防护装备工程技术研究中心,北京100071

出　　处：《中华预防医学杂志》2012年第1期67-69,共3页Chinese Journal of Preventive Medicine

摘　　要：目的评价某种正压生物防护服对病毒气溶胶的防护效果。方法制备Phi—X174噬菌体悬液，在气溶胶密闭舱室内发生Phi—X174噬菌体气溶胶，应用空气动力学粒子分析仪检测粒子直径，在高送风档和低送风档条件下，调节气溶胶密闭舱室内的湿度，用安德森六级采样器采样，通过计数噬菌体噬斑数评价该正压生物防护服对病毒气溶胶的防护效率。结果Phi-X174噬菌体气溶胶粒子质量中值直径约为0．922仙m，气溶胶粒子本底浓度〉2．0×10^4个／m^3，在不同的测试条件下，该正压生物防护服内病毒气溶胶浓度为0—21PFU／m^3，对Phi—X174噬菌体气溶胶粒子防护效率均〉99．9％，送风量（P=0．84）、环境湿度（P=0．33）以及采样时间（P=0．07）对正压防护服防护效率的影响无统计学意义。结论该正压生物防护服对Phi—X174噬菌体气溶胶的防护效果较好。Objective To evaluate the protective performance of a positive pressure bio-protective clothing against viral aerosol. Methods The suspension of indicating virus phage Phi-X174 was made for viral aerosol generating in a hermetic cabin. The diameter of viral aerosol particles were measured with a aerodynamics size analyzer. By adjusting the inner humidity of the cabin, the protective efficiency of the positive pressure bio-protecfive clothing against viral aerosol in high and low windshield conditions was determined with Andersen six-stage air sampler sampling and plage forming unit （PFU） counting, respectively. Results The mass median diameter of Phage Phi-X174 aerosol particles was about 0. 922 μm and the background concentration is beyond 2 × 10^4 particles/m^3. The protective efficiency of the clothing against phage Phi-X174 aerosol particles was above 99. 9% under different test conditions with the range of viral aerosol concentration between 0 - 23 PFU/m^3. Airflow （ P = 0. 84 ） , environment humidity conditions （P= 0. 33 ） and sampling time （P= 0. 07 ） did not affect the protective efficiency statistically. Conclusion The positive pressure bio-protective clothing provided a relatively high efficiency against phage Phi-X174 aerosol regardless of airflow rate, environment humidity and sampling time.

关 键 词：防护服 评价研究 Phi—X174噬菌体 病毒气溶胶 

分 类 号：R[医药卫生]