稻草添补百脉根体外甲烷产量的动态响应  

Effects of addition of Lotus corniculatuinto rice straw on methane emission in vitro

作　　者：李龙瑞[1,2] 张吉鹍[2,3] 邹庆华[3] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]江西新天地药业有限公司兽药研究院,江西峡江331400 [3]江西省农业科学院畜牧兽医研究所,江西南昌330200

出　　处：《草业科学》2012年第1期135-143,共9页Pratacultural Science

基　　金：国家自然科学基金(31060313);江西省科技支撑计划资助项目(2009BNA06500);反刍动物饲料安全评价项目

摘　　要：为了研究稻草添补百脉根(Lotus corniculatus)的瘤胃体外发酵及甲烷产量的动态变化,采用单因子试验设计,研究稻草与百脉根分别以100∶0、90∶10、80∶20、70∶30、60∶40、50∶50、40∶60、30∶70、20∶80、10∶90与0∶100的比例组合在3、6、12、24、36与48h的瘤胃体外的发酵参数与甲烷产量。结果表明,3～48h各组的pH值6.64～6.99,NH3-N质量浓度2.98～28.74mg.dL-1,甲烷产量0.038～1.313mmol。24h前甲烷产量随百脉根在混合发酵基质中比例的增加而增加,多数组间差异不显著(P>0.05),24～48h则随百脉根在混合发酵基质中比例的增加而减少,组间差异显著(P<0.05)。本研究结果表明,山羊(Caprasp.)稻草基础日粮添补不同水平百脉根均可以降低瘤胃体外发酵的甲烷产量。A single-factor randomized design was used to estimate the methane emission of addition of Lotus corniculatus （LC） into rice straw （RS） in vitro fermentation after 48 h by using the ratio of RS to LC with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% （LC0, LC10, LC20, LC30, LC40, LC50, I.C60, LC70, LC80, LC90 and LC100）. The results of this study showed the pH value was 6.64 to 6.99, and the NH3- N was 2.98 to 28.74 mg. dL -1, and the methane emission was0.038 to 1.313 mmol for various groups during 3--36 h. The methane emission before 24 h increased as the LC proportion increased in the mixed fermentations substrates, but it was different among groups. However, the methane emission decreased with the increase of LC proportion from 24 to 48 h and it was significantly different among groups （P〈0.05）. This study suggested that RS basic diet supplemented with LC could reduce the methane emission in vitro batch incubation system.

关 键 词：稻草 百脉根 组合 体外发酵 甲烷 

分 类 号：S816[农业科学—饲料科学]