胃癌组织中E-cadherin基因启动子异常甲基化状态观察  

Aberrant methylation of E-cadherin gene promoter in gastric carcinoma

作　　者：姜蕊[1] 赵春明[1] 宋美娟[1] 宋伟[1] 

机构地区：[1]山东大学附属省立医院,济南250021

出　　处：《山东医药》2011年第50期4-6,共3页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Z2007C18);山东省科技攻关项目(2007GG30002024)

摘　　要：目的观察胃癌组织中E-cadherin基因启动子异常甲基化状态。方法采用甲基化特异性聚合酶链反应(MSP)技术检测54例胃癌及其癌旁正常组织中的E-cadherin基因启动子异常甲基化状态,并分析E-cadherin基因启动子异常甲基化状态与胃癌临床病理参数的关系。结果胃癌组织中的E-cadherin基因启动子异常甲基化发生频率为48.10%(26/54),明显高于癌旁正常组织中的11.11%(6/54)。E-cadherin基因启动子异常甲基化频率与胃癌分化程度、病理类型、浸润深度以及淋巴结转移、临床分期有关(P均<0.05)。结论胃癌组织中E-cadher-in基因启动子异常甲基化频率较高,并与胃癌浸润、转移有关。Objective To observe the aberrant methylation of E-cadherin gene promoter in gastric carcinoma. Methods Aberrant methylation of E-cadherin gene promoter was examined by methylation specific-PCR （MSP） in gastric carcinoma and normal gastric tissues （n = 54）. Results Aberrant methylation rate of E-cadherin gene promoter was 48.1% （26/54） in gastric carcinoma tissues and and 11.11% （6/54） in normal gastric tissues respectively （ P 〈 0.05 ）. There were significant correlation between E-cadherin gene promoter methylation rate and pathology type, clinical staging, depth of tumour invasion and lymphatic metastasis. Conclusion Methylation of E-cadherin is a common molecular event and may be related to invasion and metastasis of gastric carcinoma.

关 键 词：胃肿瘤 钙黏蛋白 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]