大肠癌组织中TAMs、TGF-β1表达与上皮间质转化的关系  

Relationship between TAMs,TGF-β1 expression and epithelial mesenchymal transition in colorectal cancer tissue

作　　者：刘宇[1] 张银旭[1] 左腾[1] 张俊华[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《山东医药》2011年第50期7-9,共3页Shandong Medical Journal

基　　金：辽宁省教育厅科研基金资助项目(LS2010102)

摘　　要：目的探讨大肠癌组织中肿瘤相关巨噬细胞(TAMs)、转化生长因子β1(TGF-β1)表达与上皮间质转化(EMT)的关系。方法采用免疫组化法检测80例大肠癌及癌旁正常组织中CD68标记的TAMs、TGF-β1、上皮标志物E-cadherin、间质标志物Vimentin蛋白。结果大肠癌组织中TAM计数为(30.6±8.2)个/HP,TGF-β1蛋白阳性58例(72.5%),E-cadherin蛋白阳性31例(38.8%),Vimentin蛋白阳性38例(47.5%);而癌旁正常组织中分别为(16.4±7.2)个/HP、23例(28.8%)、64例(80.0%)、0例。两者比较,P均<0.01。TAMs计数及TGF-β1、E-cadherin、Vimentin蛋白表达与大肠癌分化程度、浸润深度、Duke分期和淋巴结转移有关(P均<0.05)。TAMs计数、TGF-β1与E-cadherin蛋白表达呈负相关(r值分别为-0.64、-0.39,P均<0.01),而与Vimentin蛋白表达成正相关(r值分别为0.62、0.33,P均<0.05)。结论大肠癌组织中TAMs浸润及TGF-β1高表达所造成的炎症环境可能与大肠癌的EMT有关。Objective To investigate the relationship between tumor-associated macrophages （TAMs）, transforming growth factor β1 （TGF-β1） expression and epithelial mesenchymal transition（EMT） in colorectal cancer tissue. Method Immunohistochemistry was used to detected the expression of TAMs marked CD6s, TGF-β1, E-cadherin and Vimentin in 80 colorectal cancer specimens and matched adjacent non-cancerous tissue specimens. Result In colorectal cancer tissue, the TAMs count was 30.6 ± 8.2/HP, E-cadherin expression was positive in 31cases （38.8%）, TGF-β1 expression was positive in 58 cases（72.5% ）, Vimentin expression was positive in 38 cases（47.5% ）. But 16.4 ± 7.2 /HP, 64 cases （80.0%） ,23 cases （28.8%）, 0 in adjacent non-cancerous tissue. TAMs count, TGF-β1, E-cadherin and Vimentin protein expression were correlated with differentiation, depth of invasion, Duke stages and lymph node metastasis in colorectal cancer, all P 〈 0.05. TAMs count, TGF-β1 expression was negatively correlated with E-cadherin protein expression （r = - 0. 64 and - 0. 39, P 〈 0.01 ） while positive correlated with Vimentin protein expression （r = 0. 52 and 0. 33, P 〈 0.05）. Conclusion The infiltration of TAMs and TGF-β1 overexpressed caused inflammatory environment maybe involved in EMT in colorectal cancer.

关 键 词：肿瘤相关巨噬细胞 上皮间质转化 转化生长因子 钙黏附蛋白-E 波形蛋白 大肠肿瘤 

分 类 号：R735.3[医药卫生—肿瘤]