微波辅助法提取构树叶中总黄酮的工艺研究  

T Research on the Extraction of Total Flavonoids from Broussonetia papyrifera by Microwave-assisted Extraction

作　　者：顾生玖[1] 姚丽新[2] 刘建楠[1] 欧奕[3] 唐世锭 朱开梅[1] 

机构地区：[1]桂林医学院药学院,广西桂林541004 [2]广西壮族自治区南溪山医院,广西桂林541002 [3]桂林三金药业股份有限公司,广西桂林541004 [4]桂林华信制药有限公司,广西灵川541200

出　　处：《安徽农业科学》2012年第2期782-785,共4页Journal of Anhui Agricultural Sciences

基　　金：广西科学研究与技术开发计划课题(桂科攻09321056;0815005-1-17)资助;桂林市科技攻关项目(科技攻关20090106-6;20080103-5)

摘　　要：[目的]优选构树叶中总黄酮的微波提取工艺。[方法]采用单因素试验考察了微波时间、微波温度、乙醇浓度、微波功率、料液比等因素的对构树叶中总黄酮得率的影响,结合正交试验优选出最佳提取条件,采用分光光度计法测定总黄酮含量。[结果]优选出最佳工艺条件为:以乙醇为提取剂,料液比1∶12(g/ml)、乙醇浓度55%、微波时间15 min、微波功率450 W、微波温度60℃;在此条件下,构树叶总黄酮的得率为79.62 mg/g。[结论]该工艺简单可行,重复性、稳定性好,可用于构树叶中总黄酮的提取。[Objective] To optimize the extraction process of total flavonoids from Broussonetia papyrifera leaves by Microwave-assisted Extraction.[Method] Single factor test was adopted to study on the effects of microwave time,microwave temperature,ethanol concentration,microwave power,solid-liquid ratio on the extraction rate of total flavonoids from B.papyrifera leaves.[Result] The optimal extraction process was selected,which was 1∶12（g/ml） solid-liquid ratio,55% ethanol concentration,15 min microwave time,450 W microwave power,60 ℃microwave temperature,and ethanol as the solvent.Under these conditions,the extraction rate of total flavonoids from B.papyrifera leaves was 79.62 mg/g.[Conclusion] This method was simple,repeatable and stable,and could be used for the extraction of total flavonoids from B.papyrifera leaves.

关 键 词：构树叶 总黄酮 微波 提取 

分 类 号：TS201.2[轻工技术与工程—食品科学]