NOV基因对肾透明细胞癌细胞MMP表达的影响  

NOV modulates expression of MMP in clear cell renal cell carcinoma

作　　者：牛志宏[1] 张辉[1] 刘帅[1] 刘征[1] 刘晓雯[1] 吕家驹[1] 

机构地区：[1]山东大学附属省立医院泌尿外科,济南250021

出　　处：《山东大学学报（医学版）》2011年第12期30-33,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2007C067)

摘　　要：目的探讨肾母细胞瘤过表达基因(NOV)对肾透明细胞癌细胞基质金属蛋白酶(MMP)表达的影响。方法构建NOV蛋白表达真核细胞重组表达质粒pEGFP-C1-NOV,转染人肾癌细胞株786-O。通过实时定量RT-PCR的方法定量转染pEGFP-C1-NOV细胞(实验组)、转染空载体细胞(空载组)和未转染的786-O细胞(空白组)的基质金属蛋白酶(MMP)亚型(包括MMP-1、MMP-2、MMP-3、MMP-7、MMP-9、MMP-13)的表达水平。方差分析法比较实验组与空载组及空白组各MMP亚型表达水平的差异。结果实验组与空白组和空载组比较,MMP-1、MMP-3和MMP-13表达水平升高(P<0.05),而MMP-2和MMP-7的表达水平下降(P<0.05)。实验组MMP-9的表达水平与空白组和空载组差异无统计学意义(P>0.05)。结论 NOV可调节肾透明细胞癌细胞MMP的表达,NOV对MMP的调节可能是NOV发挥功能的机制之一。Objective To investigate the effect of the nephroblastoma over-expressed（NOV） gene on expression of matrix metalloproteinase（MMP） in clear cell renal cell carcinoma（RCC）.Methods A NOV expression plasmid was constructed,then the plasmid was transfected into the clear cell RCC cell line 786-O,and the effect of high expression of NOV on expressions of MMP-1,MMP-2,MMP-3,MMP-7,MMP-9 and MMP-13 in 786-O cells were analyzed by real-time RT-PCR assay.Results Real-time PCR showed that expressions of MMP-1,MMP-3 and MMP-13 were significantly higher in NOV-transfected cells compared with untransfected 786-O cells and empty vector-transfected cells（P0.05）.Expressions of MMP-2 and MMP-7 were significantly lower in NOV-transfected cells compared with untransfected 786-O cells and empty vector-transfected cells（P0.05）.There was no remarkable difference in expression of MMP-9 among NOV-transfected cells,untransfectd 786-O cells and empty vector-transfected cells（P0.05）.Conclusion NOV plays a role in the modulation of MMP expression in clear cell RCC.

关 键 词：肾肿瘤 肾母细胞瘤过表达基因 基质金属蛋白酶 

分 类 号：R737.11[医药卫生—肿瘤]