乳腺癌TE方案耐药株裸鼠移植瘤NE方案化疗疗效的相关性研究  

Correlation Study of NE Regimen to Xenotransplanted Breast Cancer Treated Invalidly by TE Regimen

作　　者：李迅[1] 李妍[1] 朱莉萍[1] 欧江华[1] 杨顺娥[1] 胡欣[1] 

机构地区：[1]新疆医科大学附属肿瘤医院化疗科,乌鲁木齐市830011

出　　处：《中国肿瘤临床》2011年第24期1543-1546,共4页Chinese Journal of Clinical Oncology

基　　金：新疆维吾尔自治区自然科学基金项目(编号:2009211A06)资助~~

摘　　要：目的:评测TE方案耐药的人乳腺癌肿瘤细胞移植裸鼠后,NE方案的疗效和survivin、BCRP及Her-2基因表达的关系。方法:培养对TE方案化疗无效的人乳腺癌组织原代细胞,接种于裸鼠腋下,随机分为3组,分别为生理盐水组、NE方案低剂量组及标准剂量组,处理前后分别测量裸鼠移植瘤的体积。采用RT-PCR法及Western-blot法分别检测化疗后移植瘤中survivin、BCRP及Her-2基因和蛋白的表达变化情况。结果:标准剂量组鼠移植瘤的生长抑制最为明显,肿瘤生长抑制率为74.5%,与对照组相比,NE组中survivin、BCRP及Her-2的基因和蛋白表达均显著下降(P<0.05)。结论:NE方案化疗对TE方案耐药的乳腺癌细胞均有较好抑制作用,可能与survivin、BCRP及Her-2基因的表达下调有关。Objective： To investigate the effect of NE regimen to xenotransplant breast cancer, which has been erroneously treated by TE regimen, and to detect the expression of surviving mRNA, BCRP mRNA, and HER-2 mRNA. Methods： The breast cancer cells were inoculated into nude mice, which were grouped and treated with 0.9% NaC1, low dose and standard dose （ NVB combination with EPI ）. The excised tumor masses were detected on the 21 st day after administration by reverse transcription polymerase chain reaction （ RT-PCR ） and Western blot to investigate the expression of surviving mRNA, BCRP mRNA, and HER-2 mRNA. Results： The standard dose group showed the most significant effect in all groups （ 74.5% ）. The expression of surviving mRNA, BCRP mRNA, and HER-2 mRNA in the standard dose group was lower than that in the low dose group （ P 〈 0.05 ）. Conclusion： NE regimen was effective to most patients who were erroneously treated by TE regimen. It was related to the down-regulated expression of survivin mRNA, BCRP mRNA, and HER-2 mRNA.

关 键 词：乳腺癌 裸鼠 移植瘤 长春瑞滨 多西紫杉醇 Survivin BCRP Her-2 

分 类 号：R737.9[医药卫生—肿瘤]