基因芯片技术及其在骨肉瘤耐药机制研究中的应用  

Gene Chip Technology and Its Use in the Research on Drug Resistance Mechanism of Osteosarcoma

作　　者：周宏明[1] 王玉学[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院骨外科,哈尔滨市150081

出　　处：《中国肿瘤临床》2011年第24期1608-1611,共4页Chinese Journal of Clinical Oncology

摘　　要：基因芯片技术打破了以往陈旧的研究模式,从系统宏观地研究生物整体基因的表达及功能。骨肉瘤患者化疗过程中肿瘤细胞对化疗药物产生耐药性是引起化疗失败的重要原因之一,其机制复杂,由肿瘤的综合特性决定,对药物的摄取减少和外排增多、DNA损伤修复能力的增强、抗调亡潜能的提高、细胞膜通透性的降低和相关酶活性的变化等都会导致骨肉瘤细胞对化疗药物产生耐药性。利用基因芯片技术从基因水平对骨肉瘤耐药机制进行研究,可以快速检测大量耐药相关基因,作为深入研究骨肉瘤化疗耐药机制有效手段。本文分别对基因芯片的原理、分类和特点,骨肉瘤耐药机制和基因芯片技术在其中的应用进行综述。Microarray （gene chip） technology has broken the old research model and has been used to study the system of graduate macro expression and function of the object. This process is possible through integrated molecular biology, laser chemistry, computer science, and other disciplines related to technology. Gene chip has a high throughput, high sensitivity, high specificity, and other parallel characteristics. Osteosarcoma is one of the most common malignant bone tumors. In the last 20 to 30 years, the treatment of osteosarcoma has made significant progress. A number of studies have shown that integrated chemotherapy can improve the survival rate of patients with osteosarcoma from 20%-30% to 60-70%. Therefore, palliative chemotherapy is no longer a passive treatment, but an im- portant way to reduce tumor recurrence rate, improve the quality of life, and prolong survival. However, the resistance of osteosarcoma cells to chemotherapeutic drug is one of important reasons for chemotherapy failure. Drug resistance is a complicated mechanism, which is decided by the integrated features of tumors, such as the reduction in drug uptake, increase in efflux, DNA damage and repair capabilities, potential to improve resistance to apoptosis, cell membrane permeability reduction, and associated changes in enzyme activity. Using technology to study the mechanism of resistance from the gene level of osteosarcoma can quickly get a large number of resistant genes. Further study of the relationship between resistant genes and the discovery of new resistant genes may provide favorable clues. Thus, gene chip technology can be used as an effective method to study the mechanisms of chemotherapy resistance in osteosarcoma. The current paper discusses the gene chip principle, classification, and characteristics of the resistance mechanisms of osteosarcoma, as well as the applications ofgene chip technology.

关 键 词：骨肉瘤 化疗 耐药 基因芯片 

分 类 号：R738[医药卫生—肿瘤]