脓毒血症患者外周血Th17细胞与细胞因子表达的变化  

Alteration ofThl7 Cells and Plasma Cytokines Levels in Sepsis Patients

作　　者：吴铁军[1] 亢翠翠[1] 

机构地区：[1]山东聊城市人民医院ICU,252000

出　　处：《麻醉与监护论坛》2011年第6期462-464,共3页Forum of Anesthesia and Monitoring

摘　　要：目的：监测脓毒症患者外周血Th17细胞和细胞因子IL-17、IL-6的表达水平．探讨脓毒血症患者免疫功能变化的可能机制。方法：取2010年4月至11月住我院ICU病房的脓毒症患者62例。采用流式细胞术检测外周血Th17％（Th17／CD4＋T）。应用ELISA（酶联免疫吸附方法）检测血浆IL-17和IL-6的表达水平。同时选取来自我院查体中心的健康查体者30例作为对照组。结果：脓毒症患者外周血Th17％明显高于健康对照组[（6．94±1.95）比（1.91±0，32）％，P〈0．01]；血浆IL-17，IL-6的表然亦最著高于对照组[（56．52±16．06）pg／ml比（19．59±1．98）pg／ml；（220，68±86．41）pg／ml比（24.39±0,87）Pg／ml]。患者血浆IL-17与IL-6表达水平里正相关（r=0．886．p〈0.01）。Th17的表达与IL-17、IL-6的表达亦呈正相关，（r1=20．846,r2=0．891．均P〈0．01）.结论：脓毒血症患者外周血中Th17％增加．血浆IL-17及IL-6表达水平亦升高．提示Th17可能参与脓毒症的发病机制。Objective： To investigate the alteration of Th 17 and its associated cytokines IL-17 and I L-6 in patients with sepsis, and their impact on immune function. Method： Sixty-two patients admitted to ICU of our hospital in April to November 2010,The frequencies of Th17 in peripheral blood were detected by flow cytometric analysis.Th17 related plasma cytokines（including IL-17 and IL-6）concentrations were measured by enzyme linked immuno-sorbent assay. Thirty healthy individuals that come from the center for examination of our hospital served as controls. Result： The frequencies of Th17 in peripheral blood was significant higher than normal control[ （6.94±1.95） %vs （1.91±0.32） %, P〈0.01].Th17 related cytokines IL-17and I L-6 markedly increased in patients with sepsis compared with normal control [ （56.52±16.06） pg/ml vs （19.59±1.98） pg/ml; ~220.68±86.41 ） pg/ml vs （24.39±0.87） pg/ml]. Plasma concentration of IL-17 was positively correlated with concentration of IL-6（r=0.886,P〈0.01）.A significant correlation was found between Th17 and IL-17, IL-6 （r1=0.846, r2=0.891, both P〈0.01 ） . Conclusion： The frequencies of Th17 increased in patients with sepsis, and its related cytokines IL-17 and IL-6 were also increased, which may suggest a potential role for Th17 in patients of sepsis.

关 键 词：脓毒血症 TH17 IL-17 IL-6 

分 类 号：R572[医药卫生—消化系统]